- Catalog number
- EK1434
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cadherin-1, also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin, is a protein that in humans is encoded by the CDH1 gene. E-cadherin is a Ca (2+) -dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. It plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 284 297 279 284 7.56 2.6% Sample 2 1708 1726 1809 1726 1742 39.23 2.2% Sample 3 5563 5211 5926 5398 5524 263.13 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 284 297 279 284 7.56 2.6% Sample 1 278 284 297 279 284 7.56 2.6% Sample 2 1708 1726 1809 1726 1742 39.23 2.2% Sample 2 1708 1726 1809 1726 1742 39.23 2.2% Sample 3 5563 5211 5926 5398 5524 263.13 4.7% Sample 3 5563 5211 5926 5398 5524 263.13 4.7%
- Synonyms
- Cadherin-1; Epithelial cadherin; E-cadherin; Uvomorulin; CD324; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; Cdh1
- Gene Name
- Cdh1
- Gene ID
- 83502
- UniProt
- Q9R0T4
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q24-S704
- Applications
- ELISA
- Field of Research
- Cadherins, Calcium Binding Proteins, Calcium Signaling, Cancer, Cell Adhesion, Cytoskeleton/ECM, Invasion/Microenvironment, Signal Transduction, Signaling Pathway
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1708 5563 288 1866 5045 Standard deviation 21.4 122.97 379.5%8 27.36 169.8 363.24 CV (%) 7.7% 7.2% 6.8% 9.5% 9.1% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1708 5563 288 1866 5045 Standard deviation 21.4 122.97 379.5%8 27.36 169.8 363.24 CV (%) 7.7% 7.2% 6.8% 9.5% 9.1% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 278 1708 5563 288 1866 5045 Mean (pg/ml) 278 1708 5563 288 1866 5045 Standard deviation 21.4 122.97 379.5%8 27.36 169.8 363.24 Standard deviation 21.4 122.97 379.5%8 27.36 169.8 363.24 CV (%) 7.7% 7.2% 6.8% 9.5% 9.1% 7.2% CV (%) 7.7% 7.2% 6.8% 9.5% 9.1% 7.2%
- Sensitivity
- <15 pg/mL
- Function
- Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 98715 MW
- Specificity
- Natural and recombinant rat E-Cadherin
- Anticoagulant
- Heparin
- Gene Name Synonym
- Cadherin-1
- Subcellular Location
- Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome . Golgi apparatus, trans-Golgi network . Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane (By similarity) . .
- Protein Name
- Cadherin-1
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
