Mouse BMP-7 ELISA Kit PicoKine®

About this product

Mouse BMP-7 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Bmp7 in bone tissue, cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1443

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-β superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 84 93 90 87 4.43 5% Sample 2 219 249 237 227 233 11.22 4.8% Sample 3 964 880 981 967 948 39.78 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 84 93 90 87 4.43 5% Sample 1 82 84 93 90 87 4.43 5% Sample 2 219 249 237 227 233 11.22 4.8% Sample 2 219 249 237 227 233 11.22 4.8% Sample 3 964 880 981 967 948 39.78 4.1% Sample 3 964 880 981 967 948 39.78 4.1%

Synonyms

Bone morphogenetic protein 7; BMP-7; Osteogenic protein 1; OP-1; Bmp7; Bmp-7, Op1

Gene Name

Bmp7

Gene ID

12162

UniProt

P23359

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: S292-H430

Applications

ELISA

Field of Research

Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Cytoskeleton/ECM, Developmental Biology, Extracellular Matrix, Growth Factors/Hormones, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Metabolic Signaling Pathways, Metabolism, Nervous System Development, Organogenesis, Pathways and Processes, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, Structures, TGF Beta

Standard

10 ng/Tube

Sample Type

Bone tissue, cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 219 964 87 198 895 Standard deviation 4.83 14.45 74.22 6.78 13.46 84.13 CV (%) 7.7%% 6.6% 7.7% 7.8% 6.8% 9.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 219 964 87 198 895 Standard deviation 4.83 14.45 74.22 6.78 13.46 84.13 CV (%) 7.7%% 6.6% 7.7% 7.8% 6.8% 9.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 82 219 964 87 198 895 Mean (pg/ml) 82 219 964 87 198 895 Standard deviation 4.83 14.45 74.22 6.78 13.46 84.13 Standard deviation 4.83 14.45 74.22 6.78 13.46 84.13 CV (%) 7.7%% 6.6% 7.7% 7.8% 6.8% 9.4% CV (%) 7.7%% 6.6% 7.7% 7.8% 6.8% 9.4%

Sensitivity

<10 pg/mL

Function

Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

49199 MW

Specificity

Natural and recombinant mouse BMP-7

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Bone morphogenetic protein 7

Subcellular Location

Secreted .

Protein Name

Bone morphogenetic protein 7

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.113 0.173 0.278 0.443 0.816 1.359 2.231 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.113 0.173 0.278 0.443 0.816 1.359 2.231 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.113 0.173 0.278 0.443 0.816 1.359 2.231 O.D. 0.032 0.113 0.173 0.278 0.443 0.816 1.359 2.231

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.