- Catalog number
- EK1444
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-β superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 94 87 84 87 3.76 4.3% Sample 2 190 232 197 213 208 16.17 7.7% Sample 3 752 848 801 765 791 37.23 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 94 87 84 87 3.76 4.3% Sample 1 86 94 87 84 87 3.76 4.3% Sample 2 190 232 197 213 208 16.17 7.7% Sample 2 190 232 197 213 208 16.17 7.7% Sample 3 752 848 801 765 791 37.23 4.7% Sample 3 752 848 801 765 791 37.23 4.7%
- Synonyms
- Bone morphogenetic protein 7 ; Protein Bmp7 ; Bmp7 ; rCG_40866
- Gene Name
- Bmp7
- Gene ID
- 85272
- UniProt
- G3V6W8
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: S292-H430
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Cytoskeleton/ECM, Developmental Biology, Extracellular Matrix, Growth Factors/Hormones, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Metabolic Signaling Pathways, Metabolism, Nervous System Development, Organogenesis, Pathways and Processes, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, Structures, TGF Beta
- Standard
- 10 ng/Tube
- Sample Type
- Bone tissue, cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 86 190 752 93 186 766 Standard deviation 4.38 14.44 36.09 5.76 16.55 49.79 CV (%) 6.5% 7.6% 4.8% 6.2% 8.9% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 86 190 752 93 186 766 Standard deviation 4.38 14.44 36.09 5.76 16.55 49.79 CV (%) 6.5% 7.6% 4.8% 6.2% 8.9% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 86 190 752 93 186 766 Mean (pg/ml) 86 190 752 93 186 766 Standard deviation 4.38 14.44 36.09 5.76 16.55 49.79 Standard deviation 4.38 14.44 36.09 5.76 16.55 49.79 CV (%) 6.5% 7.6% 4.8% 6.2% 8.9% 6.5% CV (%) 6.5% 7.6% 4.8% 6.2% 8.9% 6.5%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 49199 MW
- Specificity
- Natural and recombinant rat BMP-7
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Bone morphogenetic protein 7
- Subcellular Location
- Secreted .
- Protein Name
- Bone morphogenetic protein 7
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.051 0.083 0.148 0.277 0.561 0.97 1.886 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.051 0.083 0.148 0.277 0.561 0.97 1.886 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.051 0.083 0.148 0.277 0.561 0.97 1.886 O.D. 0.014 0.051 0.083 0.148 0.277 0.561 0.97 1.886
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
