Human IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine®

About this product

Human IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine® (96 Tests) . Quantitate Human IDS in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1452

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 336 299 345 321 325 17.41 5.3% Sample 2 1290 1156 1326 1367 1284 79.16 6.1% Sample 3 4175 4541 4703 4646 4516 205.41 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 336 299 345 321 325 17.41 5.3% Sample 1 336 299 345 321 325 17.41 5.3% Sample 2 1290 1156 1326 1367 1284 79.16 6.1% Sample 2 1290 1156 1326 1367 1284 79.16 6.1% Sample 3 4175 4541 4703 4646 4516 205.41 4.5% Sample 3 4175 4541 4703 4646 4516 205.41 4.5%

Synonyms

Iduronate 2-sulfatase;3.1.6.13; Alpha-L-iduronate sulfate sulfatase; Idursulfase; Iduronate 2-sulfatase 42 kDa chain; Iduronate 2-sulfatase 14 kDa chain; IDS; SIDS

Gene Name

IDS

Gene ID

3423

UniProt

P22304

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S26-P550

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Liver, kidney, lung, and placenta.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 336 1290 4175 344 1377 4351 Standard deviation 22.84 54.18 324.25 27.86 68.85 417.69 CV (%) 6.8% 4.2% 7.8% 8.1% 5% 9.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 336 1290 4175 344 1377 4351 Standard deviation 22.84 54.18 324.25 27.86 68.85 417.69 CV (%) 6.8% 4.2% 7.8% 8.1% 5% 9.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 336 1290 4175 344 1377 4351 Mean (pg/ml) 336 1290 4175 344 1377 4351 Standard deviation 22.84 54.18 324.25 27.86 68.85 417.69 Standard deviation 22.84 54.18 324.25 27.86 68.85 417.69 CV (%) 6.8% 4.2% 7.8% 8.1% 5% 9.6% CV (%) 6.8% 4.2% 7.8% 8.1% 5% 9.6%

Sensitivity

<15 pg/mL

Function

Required for the lysosomal degradation of heparan sulfate and dermatan sulfate.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

61873 MW

Specificity

Natural and recombinant human IDS

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Iduronate 2-sulfatase

Subcellular Location

Lysosome.

Protein Name

Iduronate 2-sulfatase

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.034 0.059 0.119 0.277 0.68 1.642 2.417 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.034 0.059 0.119 0.277 0.68 1.642 2.417 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.034 0.059 0.119 0.277 0.68 1.642 2.417 O.D. 0.005 0.034 0.059 0.119 0.277 0.68 1.642 2.417

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.