- Catalog number
- EK0771
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Kunitz-type protease inhibitor 1 is an enzyme that in humans is encoded by the SPINT1 gene. It is a member of the Kunitz family of serine protease inhibitors. The SPINT1 gene encodes an inhibitor of hepatocyte growth factor activator (HGFAC) and matriptase and is involved in degradation of the extracellular matrix. SPINT1 is produced as an active membrane-associated protein and is proteolytically cleaved and secreted. Alternative splicing results in multiple variants encoding different isoforms.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 222 251 218 240 232 13.4 5.7% Sample 2 1803 1701 1702 1716 1730 42.27 2.4% Sample 3 3990 4390 4302 4668 4337 241.86 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 222 251 218 240 232 13.4 5.7% Sample 1 222 251 218 240 232 13.4 5.7% Sample 2 1803 1701 1702 1716 1730 42.27 2.4% Sample 2 1803 1701 1702 1716 1730 42.27 2.4% Sample 3 3990 4390 4302 4668 4337 241.86 5.5% Sample 3 3990 4390 4302 4668 4337 241.86 5.5%
- Synonyms
- Kunitz-type protease inhibitor 1; Hepatocyte growth factor activator inhibitor type 1; HAI-1; SPINT1; HAI1; UNQ223/PRO256
- Gene Name
- SPINT1
- Gene ID
- 6692
- UniProt
- O43278
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: G36-L529
- Applications
- ELISA
- Field of Research
- Cell Biology, Protease Inhibitors, Proteolysis/Ubiquitin
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 222 1803 3990 241 1873 3890 Standard deviation 14.65 108.18 251.37 19.03 123.61 303.42 CV (%) 6.6% 6% 6.6% 7.9% 6.6% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 222 1803 3990 241 1873 3890 Standard deviation 14.65 108.18 251.37 19.03 123.61 303.42 CV (%) 6.6% 6% 6.6% 7.9% 6.6% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 222 1803 3990 241 1873 3890 Mean (pg/ml) 222 1803 3990 241 1873 3890 Standard deviation 14.65 108.18 251.37 19.03 123.61 303.42 Standard deviation 14.65 108.18 251.37 19.03 123.61 303.42 CV (%) 6.6% 6% 6.6% 7.9% 6.6% 7.8% CV (%) 6.6% 6% 6.6% 7.9% 6.6% 7.8%
- Sensitivity
- <15 pg/mL
- Function
- Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14) .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 58398 MW
- Specificity
- Natural and recombinant human SPINT1
- Anticoagulant
- Heparin
- Gene Name Synonym
- Kunitz-type protease inhibitor 1
- Subcellular Location
- Secreted.
- Protein Name
- Kunitz-type protease inhibitor 1
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.094 0.144 0.256 0.461 0.927 1.689 2.436 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.094 0.144 0.256 0.461 0.927 1.689 2.436 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.094 0.144 0.256 0.461 0.927 1.689 2.436 O.D. 0.038 0.094 0.144 0.256 0.461 0.927 1.689 2.436
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
