- Catalog number
- EK1546
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Basal cell adhesion molecule is a protein that in humans is encoded by the BCAM gene. The BCAM gene encodes a glycoprotein expressed on cell surfaces. It is a member of the immunoglobulin superfamily of receptors and cell adhesion molecules. When expressed on erythrocytes, BCAM constitutes the Lutheran (Lu) and Auberger (Au) blood group antigens. And this protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 210 181 205 206 200 11.41 5.7% Sample 2 1405 1288 1480 1260 1358 88.87 6.5% Sample 3 5441 6068 5199 5671 5594 320.17 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 210 181 205 206 200 11.41 5.7% Sample 1 210 181 205 206 200 11.41 5.7% Sample 2 1405 1288 1480 1260 1358 88.87 6.5% Sample 2 1405 1288 1480 1260 1358 88.87 6.5% Sample 3 5441 6068 5199 5671 5594 320.17 5.7% Sample 3 5441 6068 5199 5671 5594 320.17 5.7%
- Synonyms
- Basal cell adhesion molecule; Auberger B antigen; B-CAM cell surface glycoprotein; F8/G253 antigen; Lutheran antigen; Lutheran blood group glycoprotein; CD239; BCAM; LU, MSK19
- Gene Name
- BCAM
- Gene ID
- 4059
- UniProt
- P50895
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E32-A547
- Tissue Specificity
- Wide tissue distribution (highest in the pancreas and very low in brain) . Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls.
- Applications
- ELISA
- Field of Research
- Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Neuroscience, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 210 1405 5441 201 1535 5023 Standard deviation 14.07 74.46 359.1 15.47 87.49 356.63 CV (%) 6.7% 5.3% 6.6% 7.7% 5.7% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 210 1405 5441 201 1535 5023 Standard deviation 14.07 74.46 359.1 15.47 87.49 356.63 CV (%) 6.7% 5.3% 6.6% 7.7% 5.7% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 210 1405 5441 201 1535 5023 Mean (pg/ml) 210 1405 5441 201 1535 5023 Standard deviation 14.07 74.46 359.1 15.47 87.49 356.63 Standard deviation 14.07 74.46 359.1 15.47 87.49 356.63 CV (%) 6.7% 5.3% 6.6% 7.7% 5.7% 7.1% CV (%) 6.7% 5.3% 6.6% 7.7% 5.7% 7.1%
- Sensitivity
- <15 pg/mL
- Function
- Laminin alpha-5 receptor. May mediate intracellular signaling. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 67405 MW
- Specificity
- Natural and recombinant human BCAM
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Basal cell adhesion molecule
- Subcellular Location
- Membrane; Single-pass type I membrane protein.
- Protein Name
- Basal cell adhesion molecule
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.07 0.122 0.234 0.45 0.909 1.536 2.249 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.07 0.122 0.234 0.45 0.909 1.536 2.249 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.07 0.122 0.234 0.45 0.909 1.536 2.249 O.D. 0.016 0.07 0.122 0.234 0.45 0.909 1.536 2.249
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
