- Catalog number
- EK1547
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Basal cell adhesion molecule is a protein that in humans is encoded by the BCAM gene. The BCAM gene encodes a glycoprotein expressed on cell surfaces. It is a member of the immunoglobulin superfamily of receptors and cell adhesion molecules. When expressed on erythrocytes, BCAM constitutes the Lutheran (Lu) and Auberger (Au) blood group antigens. And this protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 105 99 112 96 103 3.4 3.3% Sample 2 288 265 272 281 277 8.57 3.1% Sample 3 896 845 826 881 862 30.17 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 105 99 112 96 103 3.4 3.3% Sample 1 105 99 112 96 103 3.4 3.3% Sample 2 288 265 272 281 277 8.57 3.1% Sample 2 288 265 272 281 277 8.57 3.1% Sample 3 896 845 826 881 862 30.17 3.5% Sample 3 896 845 826 881 862 30.17 3.5%
- Synonyms
- Basal cell adhesion molecule; B-CAM cell surface glycoprotein; Lutheran antigen; CD239; Bcam; Gplu, Lu
- Gene Name
- Bcam
- Gene ID
- 57278
- UniProt
- Q9R069
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E26-A541
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cardiovascular, Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Neuroscience, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 288 896 112 303 922 Standard deviation 5.67 20.45 61.82 8.06 19.7 58.9 CV (%) 5.4% 7.1% 6.9% 7.2% 6.5% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 288 896 112 303 922 Standard deviation 5.67 20.45 61.82 8.06 19.7 58.9 CV (%) 5.4% 7.1% 6.9% 7.2% 6.5% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 105 288 896 112 303 922 Mean (pg/ml) 105 288 896 112 303 922 Standard deviation 5.67 20.45 61.82 8.06 19.7 58.9 Standard deviation 5.67 20.45 61.82 8.06 19.7 58.9 CV (%) 5.4% 7.1% 6.9% 7.2% 6.5% 6.3% CV (%) 5.4% 7.1% 6.9% 7.2% 6.5% 6.3%
- Sensitivity
- <15 pg/mL
- Function
- Laminin alpha-5 receptor. May mediate intracellular signaling (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 67670 MW
- Specificity
- Natural and recombinant mouse BCAM
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Basal cell adhesion molecule
- Subcellular Location
- Membrane ; Single-pass type I membrane protein .
- Protein Name
- Basal cell adhesion molecule
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.038 0.149 0.223 0.349 0.544 0.901 1.45 2.224 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.038 0.149 0.223 0.349 0.544 0.901 1.45 2.224 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.038 0.149 0.223 0.349 0.544 0.901 1.45 2.224 O.D. 0.038 0.149 0.223 0.349 0.544 0.901 1.45 2.224
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
