- Catalog number
- EK1479
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Pancreasin, also known as PRSS27 or Marapsin, is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. It belongs to the group-1 subfamily of serine proteases. The pancreasin gene is mapped to chromosome 16p13.3. Pancreasin is resistant to inactivation by large molecular mass trypsin inhibitors, but it is sensitive to benzamidine and leupeptin.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 167 176 171 165 169 4.2 2.4% Sample 2 1395 1278 1352 1276 1325 50.59 3.8% Sample 3 5662 5287 5308 6068 5581 318.07 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 167 176 171 165 169 4.2 2.4% Sample 1 167 176 171 165 169 4.2 2.4% Sample 2 1395 1278 1352 1276 1325 50.59 3.8% Sample 2 1395 1278 1352 1276 1325 50.59 3.8% Sample 3 5662 5287 5308 6068 5581 318.07 5.6% Sample 3 5662 5287 5308 6068 5581 318.07 5.6%
- Synonyms
- Serine protease 27;3.4.21.-; Marapsin; Pancreasin; PRSS27; MPN; UNQ1884/PRO4327
- Gene Name
- PRSS27
- Gene ID
- 83886
- UniProt
- Q9BQR3
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: M35-K290
- Tissue Specificity
- Expressed predominantly in the pancreas. .
- Applications
- ELISA
- Field of Research
- Cell Biology, Proteolysis/Ubiquitin, Proteolytic Enzymes
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 125 pg/mL - 8,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 167 13161 5662 161 1417 5245 Standard deviation 10.35 58.59 311.41 12.39 77.93 326.2%9 CV (%) 6.2% 4.2% 5.5% 7.7% 5.5% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 167 13161 5662 161 1417 5245 Standard deviation 10.35 58.59 311.41 12.39 77.93 326.2%9 CV (%) 6.2% 4.2% 5.5% 7.7% 5.5% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 167 13161 5662 161 1417 5245 Mean (pg/ml) 167 13161 5662 161 1417 5245 Standard deviation 10.35 58.59 311.41 12.39 77.93 326.2%9 Standard deviation 10.35 58.59 311.41 12.39 77.93 326.2%9 CV (%) 6.2% 4.2% 5.5% 7.7% 5.5% 6.2% CV (%) 6.2% 4.2% 5.5% 7.7% 5.5% 6.2%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 31940 MW
- Specificity
- Natural and recombinant human Marapsin
- Gene Name Synonym
- Serine protease 27
- Subcellular Location
- Secreted.
- Protein Name
- Serine protease 27
- Sample Data
- Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.014 0.074 0.138 0.277 0.501 0.87 1.686 2.529 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.014 0.074 0.138 0.277 0.501 0.87 1.686 2.529 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.014 0.074 0.138 0.277 0.501 0.87 1.686 2.529 O.D. 0.014 0.074 0.138 0.277 0.501 0.87 1.686 2.529
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
