Mouse Marapsin/Pancresin ELISA Kit PicoKine®

About this product

Mouse Marapsin/Pancresin ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Prss27 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1481

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Pancreasin, also known as PRSS27 or Marapsin, is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. It belongs to the group-1 subfamily of serine proteases. The pancreasin gene is mapped to chromosome 16p13.3. Pancreasin is resistant to inactivation by large molecular mass trypsin inhibitors, but it is sensitive to benzamidine and leupeptin.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 178 167 199 181 181 11.49 6.3% Sample 2 1127 1176 1034 1195 1133 62.3 5.4% Sample 3 4357 5001 4832 4935 4781 252.23 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 178 167 199 181 181 11.49 6.3% Sample 1 178 167 199 181 181 11.49 6.3% Sample 2 1127 1176 1034 1195 1133 62.3 5.4% Sample 2 1127 1176 1034 1195 1133 62.3 5.4% Sample 3 4357 5001 4832 4935 4781 252.23 5.2% Sample 3 4357 5001 4832 4935 4781 252.23 5.2%

Synonyms

Serine protease 27;3.4.21.-; Marapsin; Pancreasin; Prss27; Mpn

Gene Name

Prss27

Gene ID

213171

UniProt

Q8BJR6

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M38-V328

Applications

ELISA

Field of Research

Cell Biology, Proteolysis/Ubiquitin, Proteolytic Enzymes

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

125 pg/mL - 8,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 178 1127 4357 171 1106 3990 Standard deviation 9.96 87.6%5 243.99 9.57 98.43 299.25 CV (%) 7.6%% 7.6% 5.6% 5.6% 8.9% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 178 1127 4357 171 1106 3990 Standard deviation 9.96 87.6%5 243.99 9.57 98.43 299.25 CV (%) 7.6%% 7.6% 5.6% 5.6% 8.9% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 178 1127 4357 171 1106 3990 Mean (pg/ml) 178 1127 4357 171 1106 3990 Standard deviation 9.96 87.6%5 243.99 9.57 98.43 299.25 Standard deviation 9.96 87.6%5 243.99 9.57 98.43 299.25 CV (%) 7.6%% 7.6% 5.6% 5.6% 8.9% 7.5% CV (%) 7.6%% 7.6% 5.6% 5.6% 8.9% 7.5%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

35789 MW

Specificity

Natural and recombinant mouse Marapsin

Gene Name Synonym

Serine protease 27

Subcellular Location

Secreted .

Protein Name

Serine protease 27

Sample Data

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.024 0.121 0.213 0.365 0.633 1.078 1.598 2.237 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.024 0.121 0.213 0.365 0.633 1.078 1.598 2.237 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.024 0.121 0.213 0.365 0.633 1.078 1.598 2.237 O.D. 0.024 0.121 0.213 0.365 0.633 1.078 1.598 2.237

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.