- Catalog number
- EK1458
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- FABP4, also known as aP2 (adipocyte Protein 2), encodes the fatty acid binding protein found in adipocytes. The FABP4 gene is mapped to 8q21. FABP4 functions as a cytosolic lipid chaperone in macrophages and is involved in regulating macrophage endoplasmic reticulum (ER) stress. Aditionally, FABP4 protein is expressed in HBEs and is strongly upregulated by both IL4 and IL13 and downregulated by IFN-gamma, suggesting a role in allergic airway inflammation.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 344 313 309 323 322 13.55 4.2% Sample 2 1350 1278 1457 1510 1398 90.45 6.4% Sample 3 4564 4763 4358 4101 4446 245.55 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 344 313 309 323 322 13.55 4.2% Sample 1 344 313 309 323 322 13.55 4.2% Sample 2 1350 1278 1457 1510 1398 90.45 6.4% Sample 2 1350 1278 1457 1510 1398 90.45 6.4% Sample 3 4564 4763 4358 4101 4446 245.55 5.5% Sample 3 4564 4763 4358 4101 4446 245.55 5.5%
- Synonyms
- Fatty acid-binding protein, adipocyte;3T3-L1 lipid-binding protein; Adipocyte lipid-binding protein; ALBP; Adipocyte-type fatty acid-binding protein; A-FABP; AFABP; Fatty acid-binding protein 4; Myelin P2 protein homolog; P15; P2 adipocyte protein; Protein 422; Fabp4; Ap2
- Gene Name
- Fabp4
- Gene ID
- 11770
- UniProt
- P04117
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: M1-A132
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cancer Metabolism, Cardiovascular, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Mesenchymal Stem Cells, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Pathways and Processes, Redox Metabolism, Signal Transduction, Stem Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates.
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 344 1350 4564 359 1418 4994 Standard deviation 26.83 74.25 351.42 29.07 93.58 409.5 CV (%) 7.8% 5.5% 7.7% 8.1% 6.6% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 344 1350 4564 359 1418 4994 Standard deviation 26.83 74.25 351.42 29.07 93.58 409.5 CV (%) 7.8% 5.5% 7.7% 8.1% 6.6% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 344 1350 4564 359 1418 4994 Mean (pg/ml) 344 1350 4564 359 1418 4994 Standard deviation 26.83 74.25 351.42 29.07 93.58 409.5 Standard deviation 26.83 74.25 351.42 29.07 93.58 409.5 CV (%) 7.8% 5.5% 7.7% 8.1% 6.6% 8.2% CV (%) 7.8% 5.5% 7.7% 8.1% 6.6% 8.2%
- Sensitivity
- <15 pg/mL
- Function
- Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 14650 MW
- Specificity
- Natural and recombinant mouse FABP4
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Fatty acid-binding protein, adipocyte
- Subcellular Location
- Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.
- Protein Name
- Fatty acid-binding protein, adipocyte
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.051 0.087 0.156 0.296 0.611 1.185 1.976 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.051 0.087 0.156 0.296 0.611 1.185 1.976 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.051 0.087 0.156 0.296 0.611 1.185 1.976 O.D. 0.019 0.051 0.087 0.156 0.296 0.611 1.185 1.976
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
