- Catalog number
- EK1467
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Growth hormone receptor is a protein that in humans is encoded by the GHR gene. It is mapped to chromosome 5p13.1-p12. This gene encodes a protein that is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization (the receptor may however also exist as a pre-assembled non-functional dimer) and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature (proportional dwarfism) .
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 353 349 346 304 338 19.78 5.8% Sample 2 1151 1302 1219 1248 1230 54.47 4.4% Sample 3 4591 4345 4168 3982 4271 224.72 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 353 349 346 304 338 19.78 5.8% Sample 1 353 349 346 304 338 19.78 5.8% Sample 2 1151 1302 1219 1248 1230 54.47 4.4% Sample 2 1151 1302 1219 1248 1230 54.47 4.4% Sample 3 4591 4345 4168 3982 4271 224.72 5.2% Sample 3 4591 4345 4168 3982 4271 224.72 5.2%
- Synonyms
- Growth hormone receptor; GH receptor; Somatotropin receptor; Growth hormone-binding protein; GH-binding protein; GHBP; Serum-binding protein; GHR
- Gene Name
- GHR
- Gene ID
- 2690
- UniProt
- P10912
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A27-Y264
- Tissue Specificity
- Expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 is expressed in lung, stomach and muscle. Low levels in liver.
- Applications
- ELISA
- Field of Research
- More Gpcr, Neurology Process, Neuroscience, Neurotransmission, Receptors / Channels
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 353 1151 4591 351 1140 4805 Standard deviation 20.47 66.75 192.82 25.8%7 71.82 201.81 CV (%) 5.8% 5.8% 4.2% 7.2% 6.3% 4.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 353 1151 4591 351 1140 4805 Standard deviation 20.47 66.75 192.82 25.8%7 71.82 201.81 CV (%) 5.8% 5.8% 4.2% 7.2% 6.3% 4.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 353 1151 4591 351 1140 4805 Mean (pg/ml) 353 1151 4591 351 1140 4805 Standard deviation 20.47 66.75 192.82 25.8%7 71.82 201.81 Standard deviation 20.47 66.75 192.82 25.8%7 71.82 201.81 CV (%) 5.8% 5.8% 4.2% 7.2% 6.3% 4.2% CV (%) 5.8% 5.8% 4.2% 7.2% 6.3% 4.2%
- Sensitivity
- <15 pg/mL
- Function
- Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 71500 MW
- Specificity
- Natural and recombinant human GHR
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Growth hormone receptor
- Subcellular Location
- Cell membrane; Single-pass type I membrane protein. On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway. .
- Protein Name
- Growth hormone receptor
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.059 0.138 0.197 0.313 0.586 1.005 1.722 2.248 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.059 0.138 0.197 0.313 0.586 1.005 1.722 2.248 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.059 0.138 0.197 0.313 0.586 1.005 1.722 2.248 O.D. 0.059 0.138 0.197 0.313 0.586 1.005 1.722 2.248
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
