Human TAFI/CPB2/Carboxypeptidase B2 ELISA Kit PicoKine®

About this product

Human TAFI/CPB2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CPB2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1502

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carboxypeptidase B2 (CPB2), also known as carboxypeptidase U (CPU), plasma carboxypeptidase B (pCPB) or thrombin-activatable fibrinolysis inhibitor (TAFI), is an enzyme that, in humans, is encoded by the gene CPB2. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin/thrombomodulin complex, CPB2 exhibits carboxypeptidase activity. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 234 245 212 225 229 12.1 5.2% Sample 2 1109 1258 1141 1184 1173 55.82 4.7% Sample 3 4549 4286 4769 4692 4574 184.06 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 234 245 212 225 229 12.1 5.2% Sample 1 234 245 212 225 229 12.1 5.2% Sample 2 1109 1258 1141 1184 1173 55.82 4.7% Sample 2 1109 1258 1141 1184 1173 55.82 4.7% Sample 3 4549 4286 4769 4692 4574 184.06 4% Sample 3 4549 4286 4769 4692 4574 184.06 4%

Synonyms

Carboxypeptidase B2;3.4.17.20 ; Carboxypeptidase U; CPU; Plasma carboxypeptidase B; pCPB; Thrombin-activable fibrinolysis inhibitor; TAFI; CPB2

Gene Name

CPB2

Gene ID

1361

UniProt

Q96IY4

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-V423

Tissue Specificity

Plasma; synthesized in the liver.

Applications

ELISA

Field of Research

Amino Acid Metabolism, Amino Acids, Atherosclerosis, Cardiovascular, Heart Disease, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Serum Proteins, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 234 1109 4549 248 1155 4773 Standard deviation 17.08 58.77 209.25 21.32 64.68 229.1 CV (%) 7.3% 5.3% 4.6% 8.6% 5.6% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 234 1109 4549 248 1155 4773 Standard deviation 17.08 58.77 209.25 21.32 64.68 229.1 CV (%) 7.3% 5.3% 4.6% 8.6% 5.6% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 234 1109 4549 248 1155 4773 Mean (pg/ml) 234 1109 4549 248 1155 4773 Standard deviation 17.08 58.77 209.25 21.32 64.68 229.1 Standard deviation 17.08 58.77 209.25 21.32 64.68 229.1 CV (%) 7.3% 5.3% 4.6% 8.6% 5.6% 4.8% CV (%) 7.3% 5.3% 4.6% 8.6% 5.6% 4.8%

Sensitivity

<15 pg/mL

Function

Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

48424 MW

Specificity

Natural and recombinant human TAFI

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Carboxypeptidase B2

Subcellular Location

Secreted.

Protein Name

Carboxypeptidase B2

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.049 0.083 0.143 0.285 0.523 1.037 1.878 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.049 0.083 0.143 0.285 0.523 1.037 1.878 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.049 0.083 0.143 0.285 0.523 1.037 1.878 O.D. 0.031 0.049 0.083 0.143 0.285 0.523 1.037 1.878

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.