Rat GDF5/Bmp 14 ELISA Kit PicoKine®

About this product

Rat GDF5/Bmp 14 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Gdf5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1504

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Growth/differentiation factor 5 is a protein that in humans is encoded by the GDF5 gene. GDF5 is a protein belonging to the transforming growth factor beta superfamily that is expressed in the developing central nervous system, and has a role in skeletal and joint development. It also increases the survival of neurones that respond to the neurotransmitter dopamine, and is a potential therapeutic molecule associated with Parkinson's disease.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 59 68 62 62 3.49 5.6% Sample 2 323 317 316 281 309 16.52 5.3% Sample 3 894 859 910 831 873 30.69 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 59 68 62 62 3.49 5.6% Sample 1 60 59 68 62 62 3.49 5.6% Sample 2 323 317 316 281 309 16.52 5.3% Sample 2 323 317 316 281 309 16.52 5.3% Sample 3 894 859 910 831 873 30.69 3.5% Sample 3 894 859 910 831 873 30.69 3.5%

Synonyms

Growth and differentiation factor-5 ; Gdf5 ; gdf-5

Gene Name

Gdf5

Gene ID

252835

UniProt

Q8K4X5

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A376-R495

Applications

ELISA

Field of Research

Metabolism, Mitochondrial, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 323 894 59 339 908 Standard deviation 3.84 14.53 50.06 4.72 21.01 57.2 CV (%) 6.4% 4.5% 5.6% 8% 6.2% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 323 894 59 339 908 Standard deviation 3.84 14.53 50.06 4.72 21.01 57.2 CV (%) 6.4% 4.5% 5.6% 8% 6.2% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 60 323 894 59 339 908 Mean (pg/ml) 60 323 894 59 339 908 Standard deviation 3.84 14.53 50.06 4.72 21.01 57.2 Standard deviation 3.84 14.53 50.06 4.72 21.01 57.2 CV (%) 6.4% 4.5% 5.6% 8% 6.2% 6.3% CV (%) 6.4% 4.5% 5.6% 8% 6.2% 6.3%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

7491 MW

Specificity

Natural and recombinant rat GDF5

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Growth differentiation factor 5

Subcellular Location

Secreted .

Protein Name

Growth and differentiation factor-5

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.056 0.076 0.123 0.223 0.426 0.969 1.721 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.056 0.076 0.123 0.223 0.426 0.969 1.721 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.056 0.076 0.123 0.223 0.426 0.969 1.721 O.D. 0.036 0.056 0.076 0.123 0.223 0.426 0.969 1.721

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.