- Catalog number
- EK1509
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Persephin, also known as PSPN, is a neurotrophic factor in the glial cell line-derived neurotrophic factor (GDNF) family. Neurotrophic factors are important for the proper development and maintenance of the nervous system. These factors promote neuronal survival and can prevent the neuronal degeneration associated with injury, toxin exposure, or neurodegenerative disease. This PSPN gene is mapped to chromosome 19p13.3. The encoded protein has amino acid similarity to its other family members, glial cell line-derived neurotrophic factor and neurturin. This gene product promotes the survival of ventral midbrain dopaminergic neurons in culture and prevents their degeneration after 6-hydroxydopamine treatment in vivo.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 175 184 206 197 190 11.88 6.2% Sample 2 597 671 675 618 640 33.61 5.2% Sample 3 3887 4123 4146 3946 4025 111.27 2.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 175 184 206 197 190 11.88 6.2% Sample 1 175 184 206 197 190 11.88 6.2% Sample 2 597 671 675 618 640 33.61 5.2% Sample 2 597 671 675 618 640 33.61 5.2% Sample 3 3887 4123 4146 3946 4025 111.27 2.7% Sample 3 3887 4123 4146 3946 4025 111.27 2.7%
- Synonyms
- Persephin; PSP; PSPN
- Gene Name
- PSPN
- Gene ID
- 5623
- UniProt
- O60542
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: W22-G156
- Applications
- ELISA
- Field of Research
- Growth and Development, Neurology Process, Neuroscience
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 93.7 pg/mL - 6,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 175 597 3887 176 600 4002 Standard deviation 8.4 35.82 295.41 8.62 37.8 352.17 CV (%) 4.8% 6% 7.6% 4.9% 6.3% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 175 597 3887 176 600 4002 Standard deviation 8.4 35.82 295.41 8.62 37.8 352.17 CV (%) 4.8% 6% 7.6% 4.9% 6.3% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 175 597 3887 176 600 4002 Mean (pg/ml) 175 597 3887 176 600 4002 Standard deviation 8.4 35.82 295.41 8.62 37.8 352.17 Standard deviation 8.4 35.82 295.41 8.62 37.8 352.17 CV (%) 4.8% 6% 7.6% 4.9% 6.3% 8.8% CV (%) 4.8% 6% 7.6% 4.9% 6.3% 8.8%
- Sensitivity
- <10 pg/mL
- Function
- Exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 16600 MW
- Specificity
- Natural and recombinant human Persephin
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Persephin
- Subcellular Location
- Secreted.
- Protein Name
- Persephin
- Sample Data
- Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.024 0.07 0.12 0.225 0.386 0.77 1.305 2.098 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.024 0.07 0.12 0.225 0.386 0.77 1.305 2.098 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.024 0.07 0.12 0.225 0.386 0.77 1.305 2.098 O.D. 0.024 0.07 0.12 0.225 0.386 0.77 1.305 2.098
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
