Human Fetuin B/FETUB ELISA Kit PicoKine®

About this product

Human Fetuin B ELISA Kit PicoKine® (96 Tests) . Quantitate Human FETUB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1498

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Fetuin-B is a protein that in humans is encoded by the FETUB gene. It is mapped to 3q27, where the FETUA gene is localized. The protein encoded by this gene is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and response to systemic inflammation. This protein may be secreted by cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 234 245 212 225 229 12.1 5.2% Sample 2 1109 1258 1141 1184 1173 55.82 4.7% Sample 3 4549 4286 4769 4692 4574 184.06 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 234 245 212 225 229 12.1 5.2% Sample 1 234 245 212 225 229 12.1 5.2% Sample 2 1109 1258 1141 1184 1173 55.82 4.7% Sample 2 1109 1258 1141 1184 1173 55.82 4.7% Sample 3 4549 4286 4769 4692 4574 184.06 4% Sample 3 4549 4286 4769 4692 4574 184.06 4%

Synonyms

Fetuin-B;16G2; Fetuin-like protein IRL685; Gugu; FETUB

Gene Name

FETUB

Gene ID

26998

UniProt

Q9UGM5

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: C16-P382

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Liver and testis.

Applications

ELISA

Field of Research

Cardiovascular, Serum Proteins

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 234 1109 4549 248 1155 4773 Standard deviation 17.08 58.77 209.25 21.32 64.68 229.1 CV (%) 7.3% 5.3% 4.6% 8.6% 5.6% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 234 1109 4549 248 1155 4773 Standard deviation 17.08 58.77 209.25 21.32 64.68 229.1 CV (%) 7.3% 5.3% 4.6% 8.6% 5.6% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 234 1109 4549 248 1155 4773 Mean (pg/ml) 234 1109 4549 248 1155 4773 Standard deviation 17.08 58.77 209.25 21.32 64.68 229.1 Standard deviation 17.08 58.77 209.25 21.32 64.68 229.1 CV (%) 7.3% 5.3% 4.6% 8.6% 5.6% 4.8% CV (%) 7.3% 5.3% 4.6% 8.6% 5.6% 4.8%

Sensitivity

<12 pg/mL

Function

Protease inhibitor required for egg fertilization. Required to prevent premature zona pellucida hardening before fertilization, probably by inhibiting the protease activity of ASTL, a protease that mediates the cleavage of ZP2 and triggers zona pellucida hardening (By similarity) . .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

42055 MW

Specificity

Natural and recombinant human Fetuin B

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Fetuin-B

Subcellular Location

Secreted .

Protein Name

Fetuin-B

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.037 0.071 0.098 0.158 0.289 0.537 1.066 1.9 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.037 0.071 0.098 0.158 0.289 0.537 1.066 1.9 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.037 0.071 0.098 0.158 0.289 0.537 1.066 1.9 O.D. 0.037 0.071 0.098 0.158 0.289 0.537 1.066 1.9

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.