- Catalog number
- EK1484
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Disintegrin and metalloproteinase domain-containing protein 15 is an enzyme that in humans is encoded by the ADAM15 gene. The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as an EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 107 104 107 107 2.87 2.6% Sample 2 674 753 761 757 736 36.05 4.8% Sample 3 1454 1384 1331 1442 1402 49.15 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 107 104 107 107 2.87 2.6% Sample 1 112 107 104 107 107 2.87 2.6% Sample 2 674 753 761 757 736 36.05 4.8% Sample 2 674 753 761 757 736 36.05 4.8% Sample 3 1454 1384 1331 1442 1402 49.15 3.5% Sample 3 1454 1384 1331 1442 1402 49.15 3.5%
- Synonyms
- Disintegrin and metalloproteinase domain-containing protein 15; ADAM 15;3.4.24.-; Metalloprotease RGD disintegrin protein; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15; MDC-15; Metargidin; ADAM15; MDC15
- Gene Name
- ADAM15
- Gene ID
- 8751
- UniProt
- Q13444
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: D207-T696
- Tissue Specificity
- Expressed in colon and small intestine. Expressed in airway smooth muscle and glomerular mesangial cells (at protein level) . Ubiquitously expressed. Overexpressed in atherosclerotic lesions. Constitutively expressed in cultured endothelium and smooth muscle. Expressed in chondrocytes. Expressed in airway smooth muscle and glomerular mesangial cells. .
- Applications
- ELISA
- Field of Research
- Cancer, Cell Adhesion, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Integrins, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 674 1454 110 704 1511 Standard deviation 5.04 53.24 59.61 7.04 64.06 64.97 CV (%) 4.5% 7.9% 4.1% 6.4% 9.1% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 674 1454 110 704 1511 Standard deviation 5.04 53.24 59.61 7.04 64.06 64.97 CV (%) 4.5% 7.9% 4.1% 6.4% 9.1% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 112 674 1454 110 704 1511 Mean (pg/ml) 112 674 1454 110 704 1511 Standard deviation 5.04 53.24 59.61 7.04 64.06 64.97 Standard deviation 5.04 53.24 59.61 7.04 64.06 64.97 CV (%) 4.5% 7.9% 4.1% 6.4% 9.1% 4.3% CV (%) 4.5% 7.9% 4.1% 6.4% 9.1% 4.3%
- Sensitivity
- <12 pg/mL
- Function
- Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 92959 MW
- Specificity
- Natural and recombinant human ADAM15
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Disintegrin and metalloproteinase domain-containing protein 15
- Subcellular Location
- Endomembrane system ; Single-pass type I membrane protein . Cell junction, adherens junction . Cell projection, cilium, flagellum . Cytoplasmic vesicle, secretory vesicle, acrosome . The majority of the protein is localized in a perinuclear compartment which may correspond to the trans-Golgi network or the late endosome. The pro-protein is the major detectable form on the cell surface, whereas the majority of the protein in the cell is processed (By similarity) . .
- Protein Name
- Disintegrin and metalloproteinase domain-containing protein 15
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.108 0.186 0.321 0.599 1.017 1.586 2.0 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.108 0.186 0.321 0.599 1.017 1.586 2.0 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.108 0.186 0.321 0.599 1.017 1.586 2.0 O.D. 0.039 0.108 0.186 0.321 0.599 1.017 1.586 2.0
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
