- Catalog number
- EK1518
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Serine/threonine-protein kinase receptor R3 is an enzyme that in humans is encoded by the ACVRL1 gene. It is also known as activin receptor-like kinase 1, or ALK1. This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 97 102 97 101 5.31 5.2% Sample 2 377 368 378 358 370 8.07 2.1% Sample 3 1376 1227 1133 1161 1224 94.02 7.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 97 102 97 101 5.31 5.2% Sample 1 110 97 102 97 101 5.31 5.2% Sample 2 377 368 378 358 370 8.07 2.1% Sample 2 377 368 378 358 370 8.07 2.1% Sample 3 1376 1227 1133 1161 1224 94.02 7.6% Sample 3 1376 1227 1133 1161 1224 94.02 7.6%
- Synonyms
- Serine/threonine-protein kinase receptor R3; SKR3;2.7.11.30; Activin receptor-like kinase 1; ALK-1; TGF-B superfamily receptor type I; TSR-I; ACVRL1; ACVRLK1, ALK1
- Gene Name
- ACVRL1
- Gene ID
- 94
- UniProt
- P37023
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: D22-Q118
- Applications
- ELISA
- Field of Research
- Amino Acid Metabolism, Amino Acids, Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, Vasculature
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 46.9 pg/mL - 3,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 377 1376 111 366 1411 Standard deviation 5.83 17.71 71.55 5.88 22.69 73.37 CV (%) 5.3% 4.7% 5.2% 5.3% 6.2% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 377 1376 111 366 1411 Standard deviation 5.83 17.71 71.55 5.88 22.69 73.37 CV (%) 5.3% 4.7% 5.2% 5.3% 6.2% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 110 377 1376 111 366 1411 Mean (pg/ml) 110 377 1376 111 366 1411 Standard deviation 5.83 17.71 71.55 5.88 22.69 73.37 Standard deviation 5.83 17.71 71.55 5.88 22.69 73.37 CV (%) 5.3% 4.7% 5.2% 5.3% 6.2% 5.2% CV (%) 5.3% 4.7% 5.2% 5.3% 6.2% 5.2%
- Sensitivity
- <15 pg/mL
- Function
- Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 56124 MW
- Specificity
- Natural and recombinant human ALK-1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Serine/threonine-protein kinase receptor R3
- Subcellular Location
- Cell membrane ; Single-pass type I membrane protein .
- Protein Name
- Serine/threonine-protein kinase receptor R3
- Sample Data
- Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.065 0.164 0.256 0.374 0.571 1.068 1.664 2.098 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.065 0.164 0.256 0.374 0.571 1.068 1.664 2.098 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.065 0.164 0.256 0.374 0.571 1.068 1.664 2.098 O.D. 0.065 0.164 0.256 0.374 0.571 1.068 1.664 2.098
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
