- Catalog number
- EK1529
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Intercellular adhesion molecule 5 is a protein that in humans is encoded by the ICAM5 gene. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 274 247 258 248 256 10.84 4.2% Sample 2 1241 1262 1337 1171 1252 59.17 4.7% Sample 3 4856 4750 4812 4563 4745 111.75 2.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 274 247 258 248 256 10.84 4.2% Sample 1 274 247 258 248 256 10.84 4.2% Sample 2 1241 1262 1337 1171 1252 59.17 4.7% Sample 2 1241 1262 1337 1171 1252 59.17 4.7% Sample 3 4856 4750 4812 4563 4745 111.75 2.3% Sample 3 4856 4750 4812 4563 4745 111.75 2.3%
- Synonyms
- Intercellular adhesion molecule 5; ICAM-5; Telencephalin; ICAM5; TLCN, TLN
- Gene Name
- ICAM5
- Gene ID
- 7087
- UniProt
- Q9UMF0
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A28-E570
- Tissue Specificity
- Expressed on neurons in the most rostral segment of the mammalian brain, the telencephalon.
- Applications
- ELISA
- Field of Research
- Cell Adhesion Proteins, Cell Type Markers, Membrane Proteins, Neuroscience, Tags & Cell Markers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 274 1241 4856 283 1292 5119 Standard deviation 13.97 55.84 233.08 18.39 63.3 250.83 CV (%) 4.9%% 4.5% 4.8% 6.5% 4.9% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 274 1241 4856 283 1292 5119 Standard deviation 13.97 55.84 233.08 18.39 63.3 250.83 CV (%) 4.9%% 4.5% 4.8% 6.5% 4.9% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 274 1241 4856 283 1292 5119 Mean (pg/ml) 274 1241 4856 283 1292 5119 Standard deviation 13.97 55.84 233.08 18.39 63.3 250.83 Standard deviation 13.97 55.84 233.08 18.39 63.3 250.83 CV (%) 4.9%% 4.5% 4.8% 6.5% 4.9% 4.9% CV (%) 4.9%% 4.5% 4.8% 6.5% 4.9% 4.9%
- Sensitivity
- <15 pg/mL
- Function
- ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 97116 MW
- Specificity
- Natural and recombinant human ICAM5
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Intercellular adhesion molecule 5
- Subcellular Location
- Membrane; Single-pass type I membrane protein.
- Protein Name
- Intercellular adhesion molecule 5
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.149 0.251 0.48 0.751 1.261 1.806 2.089 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.149 0.251 0.48 0.751 1.261 1.806 2.089 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.149 0.251 0.48 0.751 1.261 1.806 2.089 O.D. 0.03 0.149 0.251 0.48 0.751 1.261 1.806 2.089
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
