- Catalog number
- EK1536
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Nectin-4, also known as PVRL4, is an adhesion receptor of the immunoglobulin superfamily and functions in cell-cell adhesion. This gene is mapped to chromosome 1q23.3. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 82 86 80 80 3.96 4.9% Sample 2 209 190 207 207 203 7.69 3.7% Sample 3 857 1006 836 933 908 67.1 7.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 82 86 80 80 3.96 4.9% Sample 1 75 82 86 80 80 3.96 4.9% Sample 2 209 190 207 207 203 7.69 3.7% Sample 2 209 190 207 207 203 7.69 3.7% Sample 3 857 1006 836 933 908 67.1 7.3% Sample 3 857 1006 836 933 908 67.1 7.3%
- Synonyms
- Nectin-4; Ig superfamily receptor LNIR; Nectin cell adhesion molecule 4 ; Poliovirus receptor-related protein 4; Processed poliovirus receptor-related protein 4; NECTIN4 ; LNIR, PRR4, PVRL4
- Gene Name
- NECTIN4
- Gene ID
- 81607
- UniProt
- Q96NY8
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: G32-S349
- Tissue Specificity
- Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma. .
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA), cell lysates and urine.
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 75 209 857 70 228 777 Standard deviation 3.67 13.16 44.56 4.13 16.41 41.18 CV (%) 4.9% 7.2%% 5.2% 5.9% 7.2% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 75 209 857 70 228 777 Standard deviation 3.67 13.16 44.56 4.13 16.41 41.18 CV (%) 4.9% 7.2%% 5.2% 5.9% 7.2% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 75 209 857 70 228 777 Mean (pg/ml) 75 209 857 70 228 777 Standard deviation 3.67 13.16 44.56 4.13 16.41 41.18 Standard deviation 3.67 13.16 44.56 4.13 16.41 41.18 CV (%) 4.9% 7.2%% 5.2% 5.9% 7.2% 5.3% CV (%) 4.9% 7.2%% 5.2% 5.9% 7.2% 5.3%
- Sensitivity
- <10 pg/mL
- Function
- Seems to be involved in cell adhesion through trans- homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 55454 MW
- Specificity
- Natural and recombinant human Nectin-4
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Nectin-4
- Subcellular Location
- Cell membrane ; Single-pass type I membrane protein . Cell junction, adherens junction . Colocalizes with MLLT4 at cadherin-based adherens junctions (PubMed:11544254) .
- Protein Name
- Nectin-4
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.086 0.133 0.207 0.375 0.6251 1.118 1.902 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.086 0.133 0.207 0.375 0.6251 1.118 1.902 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.086 0.133 0.207 0.375 0.6251 1.118 1.902 O.D. 0.028 0.086 0.133 0.207 0.375 0.6251 1.118 1.902
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
