- Catalog number
- EK1538
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Kremen protein 1 is a protein that in humans is encoded by the KREMEN1 gene. This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT) /beta-catenin signaling. The encoded protein is a component of a membrane complex that modulates canonical WNT signaling through lipoprotein receptor-related protein 6 (LRP6) . It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 171 171 172 159 168 5.35 3.1% Sample 2 880 925 969 991 941 42.6 4.5% Sample 3 4010 4034 3878 4402 4081 194.61 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 171 171 172 159 168 5.35 3.1% Sample 1 171 171 172 159 168 5.35 3.1% Sample 2 880 925 969 991 941 42.6 4.5% Sample 2 880 925 969 991 941 42.6 4.5% Sample 3 4010 4034 3878 4402 4081 194.61 4.7% Sample 3 4010 4034 3878 4402 4081 194.61 4.7%
- Synonyms
- Kremen protein 1; Dickkopf receptor; Kringle domain-containing transmembrane protein 1; Kringle-containing protein marking the eye and the nose; Kremen1; Kremen
- Gene Name
- Kremen1
- Gene ID
- 84035
- UniProt
- Q99N43
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A20-G395
- Tissue Specificity
- In the adult, widely expressed with high levels in heart, lung, kidney, skeletal muscle and testis. .
- Applications
- ELISA
- Field of Research
- Cadherins, Cell Adhesion, Cytoskeleton/ECM, Signal Transduction, Signaling Pathways, Stem Cells, Surface Molecules
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 125 pg/mL - 8,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 171 880 4010 187 831 3851 Standard deviation 6.84 57.2 308.77 8.41 59 323.48 CV (%) 4% 6.5% 7.7% 4.5% 7.1% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 171 880 4010 187 831 3851 Standard deviation 6.84 57.2 308.77 8.41 59 323.48 CV (%) 4% 6.5% 7.7% 4.5% 7.1% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 171 880 4010 187 831 3851 Mean (pg/ml) 171 880 4010 187 831 3851 Standard deviation 6.84 57.2 308.77 8.41 59 323.48 Standard deviation 6.84 57.2 308.77 8.41 59 323.48 CV (%) 4% 6.5% 7.7% 4.5% 7.1% 8.4% CV (%) 4% 6.5% 7.7% 4.5% 7.1% 8.4%
- Sensitivity
- <10 pg/mL
- Function
- Receptor for Dickkopf protein. Cooperates with Dickkopf to block Wnt/beta-catenin signaling (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 51673 MW
- Specificity
- Natural and recombinant mouse Kremen-1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Kremen protein 1
- Subcellular Location
- Membrane ; Single-pass type I membrane protein .
- Protein Name
- Kremen protein 1
- Sample Data
- Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.042 0.117 0.204 0.345 0.641 1.14 1.612 2.125 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.042 0.117 0.204 0.345 0.641 1.14 1.612 2.125 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.042 0.117 0.204 0.345 0.641 1.14 1.612 2.125 O.D. 0.042 0.117 0.204 0.345 0.641 1.14 1.612 2.125
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
