- Catalog number
- EK0774
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Kunitz-type protease inhibitor 2 is an enzyme inhibitor that in humans is encoded by the SPINT2 gene. This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. And this gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 61 62 57 60 1.92 3.2% Sample 2 240 243 264 249 249 9.24 3.7% Sample 3 837 921 842 963 890 53.38 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 61 62 57 60 1.92 3.2% Sample 1 61 61 62 57 60 1.92 3.2% Sample 2 240 243 264 249 249 9.24 3.7% Sample 2 240 243 264 249 249 9.24 3.7% Sample 3 837 921 842 963 890 53.38 5.9% Sample 3 837 921 842 963 890 53.38 5.9%
- Synonyms
- Kunitz-type protease inhibitor 2; Hepatocyte growth factor activator inhibitor type 2; HAI-2; Spint2; Hai2
- Gene Name
- Spint2
- Gene ID
- 20733
- UniProt
- Q9WU03
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: R30-K140
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Isoform 2 is more predominantly expressed than isoform 1.
- Applications
- ELISA
- Field of Research
- Cell Biology, Developmental Biology, Neurogenesis, Neurology Process, Neuroscience, Protease Inhibitors, Proteolysis/Ubiquitin, Reproduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates.
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 240 837 64 248 772 Standard deviation 4.14 13.92 46.03 5.56 17.85 55.58 CV (%) 6.8% 5.8% 5.5% 8.7% 7.2% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 240 837 64 248 772 Standard deviation 4.14 13.92 46.03 5.56 17.85 55.58 CV (%) 6.8% 5.8% 5.5% 8.7% 7.2% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 61 240 837 64 248 772 Mean (pg/ml) 61 240 837 64 248 772 Standard deviation 4.14 13.92 46.03 5.56 17.85 55.58 Standard deviation 4.14 13.92 46.03 5.56 17.85 55.58 CV (%) 6.8% 5.8% 5.5% 8.7% 7.2% 7.2% CV (%) 6.8% 5.8% 5.5% 8.7% 7.2% 7.2%
- Sensitivity
- <10 pg/mL
- Function
- Inhibitor of HGF activator.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 27914 MW
- Specificity
- Natural and recombinant mouse HAI-2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Kunitz-type protease inhibitor 2
- Subcellular Location
- Membrane ; Single-pass type I membrane protein .
- Protein Name
- Kunitz-type protease inhibitor 2
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.172 0.245 0.391 0.618 1.017 1.57 2.395 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.172 0.245 0.391 0.618 1.017 1.57 2.395 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.172 0.245 0.391 0.618 1.017 1.57 2.395 O.D. 0.036 0.172 0.245 0.391 0.618 1.017 1.57 2.395
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
