Human AGP1/alpha 1 acid glycoprotein ELISA Kit PicoKine®

About this product

Human AGP1/alpha 1 acid glycoprotein ELISA Kit PicoKine® (96 Tests) . Quantitate Human ORM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 150pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1486

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Alpha-1-acid glycoprotein, or orosomucoid, is a relatively abundant plasma protein synthesized in the liver. It has a molecular weith of 40,000 Da and is a single polypeptide chain of about 180 amino acids. AGP is considered an acute phase reactant; its plasma levels increase several fold during acute infections. It belongs to the lipocalin family and binds numerous basic and neutral lipophilic drugs and steroid hormones. And this gene is located in 9q34.3-qter.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2696 2424 2599 2810 2632 141.53 5.3% Sample 2 12095 11209 12283 11042 11657 539.13 4.6% Sample 3 38792 45455 38596 41095 40984 2761.77 6.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2696 2424 2599 2810 2632 141.53 5.3% Sample 1 2696 2424 2599 2810 2632 141.53 5.3% Sample 2 12095 11209 12283 11042 11657 539.13 4.6% Sample 2 12095 11209 12283 11042 11657 539.13 4.6% Sample 3 38792 45455 38596 41095 40984 2761.77 6.7% Sample 3 38792 45455 38596 41095 40984 2761.77 6.7%

Synonyms

Alpha-1-acid glycoprotein 1; AGP 1; Orosomucoid-1; OMD 1; ORM1; AGP1

Gene Name

ORM1

Gene ID

5004

UniProt

P02763

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q19-S201

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed by the liver and secreted in plasma.

Applications

ELISA

Field of Research

Cardiovascular, Immunology, Innate Immunity, Macrophage/Inflammation, Secreted Molecules, Serum Proteins

Standard

100 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

1.56 ng/mL - 100 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2696 12095 38792 2753 12889 38542 Standard deviation 164.45 931.31 1784.43 192.71 1198.67 2235.43 CV (%) 6.1% 7.7% 4.6% 7% 9.3% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2696 12095 38792 2753 12889 38542 Standard deviation 164.45 931.31 1784.43 192.71 1198.67 2235.43 CV (%) 6.1% 7.7% 4.6% 7% 9.3% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2696 12095 38792 2753 12889 38542 Mean (pg/ml) 2696 12095 38792 2753 12889 38542 Standard deviation 164.45 931.31 1784.43 192.71 1198.67 2235.43 Standard deviation 164.45 931.31 1784.43 192.71 1198.67 2235.43 CV (%) 6.1% 7.7% 4.6% 7% 9.3% 5.8% CV (%) 6.1% 7.7% 4.6% 7% 9.3% 5.8%

Sensitivity

<150 pg/mL

Function

Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

23512 MW

Specificity

Natural and recombinant human AGP1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Alpha-1-acid glycoprotein 1

Subcellular Location

Secreted.

Protein Name

Alpha-1-acid glycoprotein 1

Sample Data

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.025 0.075 0.119 0.21 0.369 0.749 1.355 2.007 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.025 0.075 0.119 0.21 0.369 0.749 1.355 2.007 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.025 0.075 0.119 0.21 0.369 0.749 1.355 2.007 O.D. 0.025 0.075 0.119 0.21 0.369 0.749 1.355 2.007

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.