- Catalog number
- EK1564
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF) -like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 51 47 44 47 2.54 5.4% Sample 2 179 201 178 187 186 9.2 4.9% Sample 3 730 656 670 709 691 29.62 4.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 51 47 44 47 2.54 5.4% Sample 1 46 51 47 44 47 2.54 5.4% Sample 2 179 201 178 187 186 9.2 4.9% Sample 2 179 201 178 187 186 9.2 4.9% Sample 3 730 656 670 709 691 29.62 4.2% Sample 3 730 656 670 709 691 29.62 4.2%
- Synonyms
- Wnt inhibitory factor 1; WIF-1; Wif1
- Gene Name
- Wif1
- Gene ID
- 114557
- UniProt
- Q6IN38
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: G29-W379
- Applications
- ELISA
- Field of Research
- Secreted, Signaling Pathways, Stem Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 23.4 pg/mL - 1500 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 179 730 48 172 703 Standard deviation 3.49 10.2 32.85 4.41 10.32 44.28 CV (%) 7.6% 5.7% 4.5% 9.2% 6% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 179 730 48 172 703 Standard deviation 3.49 10.2 32.85 4.41 10.32 44.28 CV (%) 7.6% 5.7% 4.5% 9.2% 6% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 46 179 730 48 172 703 Mean (pg/ml) 46 179 730 48 172 703 Standard deviation 3.49 10.2 32.85 4.41 10.32 44.28 Standard deviation 3.49 10.2 32.85 4.41 10.32 44.28 CV (%) 7.6% 5.7% 4.5% 9.2% 6% 6.3% CV (%) 7.6% 5.7% 4.5% 9.2% 6% 6.3%
- Sensitivity
- <10 pg/mL
- Function
- Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 41585 MW
- Specificity
- Natural and recombinant rat WIF1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Wnt inhibitory factor 1
- Subcellular Location
- Secreted.
- Protein Name
- Wnt inhibitory factor 1
- Sample Data
- Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.041 0.141 0.193 0.341 0.598 0.852 1.531 2.056 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.041 0.141 0.193 0.341 0.598 0.852 1.531 2.056 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.041 0.141 0.193 0.341 0.598 0.852 1.531 2.056 O.D. 0.041 0.141 0.193 0.341 0.598 0.852 1.531 2.056
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
