Rat Angiopoietin-2 ELISA Kit PicoKine®

About this product

Rat Angiopoietin-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Angpt2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1574

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 253 256 215 222 236 18.2 7.7% Sample 2 1986 2081 1801 1998 1966 102.31 5.2% Sample 3 5096 4569 4759 5117 4885 231.34 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 253 256 215 222 236 18.2 7.7% Sample 1 253 256 215 222 236 18.2 7.7% Sample 2 1986 2081 1801 1998 1966 102.31 5.2% Sample 2 1986 2081 1801 1998 1966 102.31 5.2% Sample 3 5096 4569 4759 5117 4885 231.34 4.7% Sample 3 5096 4569 4759 5117 4885 231.34 4.7%

Synonyms

Angiopoietin-2; ANG-2; Angpt2; Agpt2

Gene Name

Angpt2

Gene ID

89805

UniProt

O35462

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y19-F496

Applications

ELISA

Field of Research

Angiogenesis, Cardiovascular, Growth Factors

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 253 1986 5096 239 1814 4721 Standard deviation 19.98 99.3 229.32 22.22 121.53 231.32 CV (%) 7.9% 5% 4.5% 9.3% 6.7% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 253 1986 5096 239 1814 4721 Standard deviation 19.98 99.3 229.32 22.22 121.53 231.32 CV (%) 7.9% 5% 4.5% 9.3% 6.7% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 253 1986 5096 239 1814 4721 Mean (pg/ml) 253 1986 5096 239 1814 4721 Standard deviation 19.98 99.3 229.32 22.22 121.53 231.32 Standard deviation 19.98 99.3 229.32 22.22 121.53 231.32 CV (%) 7.9% 5% 4.5% 9.3% 6.7% 4.9% CV (%) 7.9% 5% 4.5% 9.3% 6.7% 4.9%

Sensitivity

<15 pg/mL

Function

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (By similarity) . .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

56518 MW

Specificity

Natural and recombinant rat Angiopoietin-2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Angiopoietin-2

Subcellular Location

Secreted.

Protein Name

Angiopoietin-2

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.034 0.106 0.186 0.343 0.663 1.134 1.88 2.417 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.034 0.106 0.186 0.343 0.663 1.134 1.88 2.417 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.034 0.106 0.186 0.343 0.663 1.134 1.88 2.417 O.D. 0.034 0.106 0.186 0.343 0.663 1.134 1.88 2.417

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.