Human Syndecan-3/SDC3 ELISA Kit PicoKine®

About this product

Human Syndecan-3/SDC3 ELISA Kit PicoKine® (96 Tests) . Quantitate Human SDC3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1555

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Syndecan-3 is a protein that in humans is encoded by the SDC3 gene. The protein encoded by this gene belongs to the syndecan proteoglycan family, which was assigned to human chromosome 1p35.2, and it showed that in the mouse the Synd3 gene maps to chromosome 4 near the Lmyc gene. The transfection of SDC3 resulted in the formation of long filopodia-like structures, microspikes, and varicosities in several cell lines. Besides, the gene plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Allelic variants of this gene have been associated with obesity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 237 215 203 238 223 14.87 6.6% Sample 2 1952 1913 1837 1906 1902 41.41 2.1% Sample 3 5230 5139 5248 5131 5187 52.46 1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 237 215 203 238 223 14.87 6.6% Sample 1 237 215 203 238 223 14.87 6.6% Sample 2 1952 1913 1837 1906 1902 41.41 2.1% Sample 2 1952 1913 1837 1906 1902 41.41 2.1% Sample 3 5230 5139 5248 5131 5187 52.46 1% Sample 3 5230 5139 5248 5131 5187 52.46 1%

Synonyms

Syndecan-3; SYND3; SDC3; KIAA0468

Gene Name

SDC3

Gene ID

9672

UniProt

O75056

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A41-V387

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed in the nervous system, the adrenal gland, and the spleen. .

Applications

ELISA

Field of Research

Cytoskeleton/ECM, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 237 12462 5230 246 1947 5345 Standard deviation 13.27 130.78 287.65 17.22 157.7 379.49 CV (%) 6.7% 6.7% 5.5% 7% 8.1% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 237 12462 5230 246 1947 5345 Standard deviation 13.27 130.78 287.65 17.22 157.7 379.49 CV (%) 6.7% 6.7% 5.5% 7% 8.1% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 237 12462 5230 246 1947 5345 Mean (pg/ml) 237 12462 5230 246 1947 5345 Standard deviation 13.27 130.78 287.65 17.22 157.7 379.49 Standard deviation 13.27 130.78 287.65 17.22 157.7 379.49 CV (%) 6.7% 6.7% 5.5% 7% 8.1% 7.1% CV (%) 6.7% 6.7% 5.5% 7% 8.1% 7.1%

Sensitivity

<10 pg/mL

Function

Cell surface proteoglycan that may bear heparan sulfate (By similarity) . May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

45497 MW

Specificity

Natural and recombinant human Syndecan-3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Syndecan-3

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

Syndecan-3

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.053 0.101 0.149 0.229 0.405 0.749 1.406 2.096 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.053 0.101 0.149 0.229 0.405 0.749 1.406 2.096 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.053 0.101 0.149 0.229 0.405 0.749 1.406 2.096 O.D. 0.053 0.101 0.149 0.229 0.405 0.749 1.406 2.096

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.