- Catalog number
- EK1557
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called amyloid. It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened β-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2959 2863 2697 2962 2870 107.66 3.7% Sample 2 13066 13637 15280 15579 14390 1063.69 7.3% Sample 3 40903 43039 47063 46975 44495 2634.74 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2959 2863 2697 2962 2870 107.66 3.7% Sample 1 2959 2863 2697 2962 2870 107.66 3.7% Sample 2 13066 13637 15280 15579 14390 1063.69 7.3% Sample 2 13066 13637 15280 15579 14390 1063.69 7.3% Sample 3 40903 43039 47063 46975 44495 2634.74 5.9% Sample 3 40903 43039 47063 46975 44495 2634.74 5.9%
- Synonyms
- Serum amyloid P-component; SAP;9.5S alpha-1-glycoprotein; Serum amyloid P-component (1-203) ; APCS; PTX2
- Gene Name
- APCS
- Gene ID
- 325
- UniProt
- P02743
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: H20-V223
- Tissue Specificity
- Found in serum and urine. .
- Applications
- ELISA
- Standard
- 100 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 1.56 ng/mL - 100 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 232279 13066 40903 3227 13910 44719 Standard deviation 189.37 548.77 2249.66 209.75 737.23 2906.73 CV (%) 6.4% 4.2% 5.5% 6.5% 5.3% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 232279 13066 40903 3227 13910 44719 Standard deviation 189.37 548.77 2249.66 209.75 737.23 2906.73 CV (%) 6.4% 4.2% 5.5% 6.5% 5.3% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 232279 13066 40903 3227 13910 44719 Mean (pg/ml) 232279 13066 40903 3227 13910 44719 Standard deviation 189.37 548.77 2249.66 209.75 737.23 2906.73 Standard deviation 189.37 548.77 2249.66 209.75 737.23 2906.73 CV (%) 6.4% 4.2% 5.5% 6.5% 5.3% 6.5% CV (%) 6.4% 4.2% 5.5% 6.5% 5.3% 6.5%
- Sensitivity
- <10 pg/mL
- Function
- Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 25387 MW
- Specificity
- Natural and recombinant human SAP
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Serum amyloid P-component
- Subcellular Location
- Secreted.
- Protein Name
- Serum amyloid P-component
- Sample Data
- Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.006 0.088 0.188 0.398 0.674 1.234 1.665 2.055 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.006 0.088 0.188 0.398 0.674 1.234 1.665 2.055 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.006 0.088 0.188 0.398 0.674 1.234 1.665 2.055 O.D. 0.006 0.088 0.188 0.398 0.674 1.234 1.665 2.055
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
