- Catalog number
- EK1577
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Neural cell adhesion molecule L1-like protein also known as close homolog of L1 (CHL1) is a protein that in humans is encoded by the CHL1 gene. This protein is a member of the L1 gene family of neural cell adhesion molecules. By FISH, the CHL1 gene was mapped to chromosome 3p26.1. The protein however has dynamic localisation, meaning that it has not only multiple roles in the cell, but also various locations. This protein may also play a role in the growth of certain cancers.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 218 223 245 253 234 14.63 6.2% Sample 2 1822 1615 1911 1772 1780 107.48 6% Sample 3 4674 4640 4837 4877 4757 101.7 2.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 218 223 245 253 234 14.63 6.2% Sample 1 218 223 245 253 234 14.63 6.2% Sample 2 1822 1615 1911 1772 1780 107.48 6% Sample 2 1822 1615 1911 1772 1780 107.48 6% Sample 3 4674 4640 4837 4877 4757 101.7 2.1% Sample 3 4674 4640 4837 4877 4757 101.7 2.1%
- Synonyms
- Neural cell adhesion molecule L1-like protein; Cell adhesion molecule with homology to L1CAM; Chl1-like protein; Close homolog of L1; Processed neural cell adhesion molecule L1-like protein; Chl1; Call
- Gene Name
- Chl1
- Gene ID
- 12661
- UniProt
- P70232
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E26-W1083
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed in the brain, in the cerebellum and in the spinal cord. Detected in the retina and the optic nerve. Expressed in neurons and glial cells in the central nervous system and by Schwann cells in the peripheral nervous system. .
- Applications
- ELISA
- Field of Research
- Actin Binding Proteins, Actin, etc., Cell Adhesion Proteins, Cytoskeleton, Cytoskeleton/ECM, ECM Proteins, Membrane Proteins, Microfilaments, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 218 1822 4674 207 1753 4970 Standard deviation 15.04 116.6 198.2% 17.59 143.74 238.56 CV (%) 6.9% 6.4% 4.2% 8.5% 8.2% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 218 1822 4674 207 1753 4970 Standard deviation 15.04 116.6 198.2% 17.59 143.74 238.56 CV (%) 6.9% 6.4% 4.2% 8.5% 8.2% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 218 1822 4674 207 1753 4970 Mean (pg/ml) 218 1822 4674 207 1753 4970 Standard deviation 15.04 116.6 198.2% 17.59 143.74 238.56 Standard deviation 15.04 116.6 198.2% 17.59 143.74 238.56 CV (%) 6.9% 6.4% 4.2% 8.5% 8.2% 4.8% CV (%) 6.9% 6.4% 4.2% 8.5% 8.2% 4.8%
- Sensitivity
- <10 pg/mL
- Function
- Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. Plays a role in neuronal positioning of pyramidal neurons as well as in regulation of both the number of interneurons and the efficacy of GABAergic synapses. May play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins. Recruits ANK3 to the plasma membrane. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 135074 MW
- Specificity
- Natural and recombinant mouse CHL1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Neural cell adhesion molecule L1-like protein
- Subcellular Location
- Cell membrane; Single-pass type I membrane protein. Soluble forms produced by cleavage/shedding also exist.
- Protein Name
- Neural cell adhesion molecule L1-like protein
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.056 0.109 0.214 0.398 0.717 1.241 1.829 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.056 0.109 0.214 0.398 0.717 1.241 1.829 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.056 0.109 0.214 0.398 0.717 1.241 1.829 O.D. 0.008 0.056 0.109 0.214 0.398 0.717 1.241 1.829
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
