- Catalog number
- EK0669-CN
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cadherin-2 (CDH2), also known as neural cadherin (NCAD), is a protein that in Dog Canineas is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 223 193 211 223 212 12.27 5.7% Sample 2 1172 1262 1146 1232 1203 46.18 3.8% Sample 3 4928 5617 4816 5488 5212 345.56 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 223 193 211 223 212 12.27 5.7% Sample 1 223 193 211 223 212 12.27 5.7% Sample 2 1172 1262 1146 1232 1203 46.18 3.8% Sample 2 1172 1262 1146 1232 1203 46.18 3.8% Sample 3 4928 5617 4816 5488 5212 345.56 6.6% Sample 3 4928 5617 4816 5488 5212 345.56 6.6%
- Synonyms
- Cadherin-2; CDw325; Neural cadherin; N-cadherin; CD325; CDH2; CDHN, NCAD
- Gene Name
- CDH2
- Gene ID
- 480169
- UniProt
- P19022
- Reactivity
- Canine
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: D160-A724
- Capture Antibody
- Polyclonal antibody from sheep
- Detection Antibody
- Polyclonal antibody from sheep
- Applications
- ELISA
- Field of Research
- Cadherins, Cell Adhesion, Cytoskeleton/ECM, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 223 1172 4928 308 1190 4953 Standard deviation 13.16 50.40 202.05 20.33 69.02 376.43 CV (%) 5.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 223 1172 4928 308 1190 4953 Standard deviation 13.16 50.40 202.05 20.33 69.02 376.43 CV (%) 5.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 223 1172 4928 308 1190 4953 Mean (pg/ml) 223 1172 4928 308 1190 4953 Standard deviation 13.16 50.40 202.05 20.33 69.02 376.43 Standard deviation 13.16 50.40 202.05 20.33 69.02 376.43 CV (%) 5.9% 4.3% 4.1% 6.6% 5.8% 7.6% CV (%) 5.9% 4.3% 4.1% 6.6% 5.8% 7.6%
- Sensitivity
- <20 pg/mL
- Function
- Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 99809 MW
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Cadherin-2
- Subcellular Location
- Cell membrane; Single-pass type I membrane protein.
- Protein Name
- Cadherin-2
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.062 0.111 0.164 0.277 0.465 0.878 1.653 2.594 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.062 0.111 0.164 0.277 0.465 0.878 1.653 2.594 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.062 0.111 0.164 0.277 0.465 0.878 1.653 2.594 O.D. 0.062 0.111 0.164 0.277 0.465 0.878 1.653 2.594
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
