- Catalog number
- EK0964-CN
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interferon lambda-1, also called Interleukin-29 (IL-29) is a protein that in Dog Canineas is encoded by the IL29 gene. IL-29 is a member of the helical cytokine family and is a type III interferon. This gene is mapped to 19q13.2. It is a cytokine with immunomodulatory activity. This gene may play a role in antiviral immunity. It can up-regulates MHC class I antigen expression. It is the ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 91 83 91 88 3.34 3.7% Sample 2 415 360 421 388 396 24.21 6.1% Sample 3 1566 1471 1612 1562 1552 51.12 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 91 83 91 88 3.34 3.7% Sample 1 90 91 83 91 88 3.34 3.7% Sample 2 415 360 421 388 396 24.21 6.1% Sample 2 415 360 421 388 396 24.21 6.1% Sample 3 1566 1471 1612 1562 1552 51.12 3.2% Sample 3 1566 1471 1612 1562 1552 51.12 3.2%
- Synonyms
- Interferon lambda-1; IFN-lambda-1; Cytokine Zcyto21; Interleukin-29; IL-29; IFNL1; IL29, ZCYTO21
- Gene Name
- IFNL1
- Gene ID
- 612539
- UniProt
- Q8IU54
- Reactivity
- Canine
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: G20-T200
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cytokines, Immunology, Innate Immunity, Interleukins
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 415 1566 103 448 1895 Standard deviation 3.78 22.00 72.04 4.94 28.67 138.34 CV (%) 4.2% 5.3% 4.6% 4.8% 6.4% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 415 1566 103 448 1895 Standard deviation 3.78 22.00 72.04 4.94 28.67 138.34 CV (%) 4.2% 5.3% 4.6% 4.8% 6.4% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 90 415 1566 103 448 1895 Mean (pg/ml) 90 415 1566 103 448 1895 Standard deviation 3.78 22.00 72.04 4.94 28.67 138.34 Standard deviation 3.78 22.00 72.04 4.94 28.67 138.34 CV (%) 4.2% 5.3% 4.6% 4.8% 6.4% 7.3% CV (%) 4.2% 5.3% 4.6% 4.8% 6.4% 7.3%
- Sensitivity
- <10 pg/mL
- Function
- Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN- stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type- selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 21898 MW
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interferon lambda-1
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the lambda interferon family.
- Protein Name
- Interferon lambda-1
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.07 0.119 0.208 0.4 0.736 1.252 2.03 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.07 0.119 0.208 0.4 0.736 1.252 2.03 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.07 0.119 0.208 0.4 0.736 1.252 2.03 O.D. 0.034 0.07 0.119 0.208 0.4 0.736 1.252 2.03
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
