Bovine BMP-2 ELISA Kit PicoKine®

About this product

Bovine BMP-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Bovine BMP2 in bone tissue, cell culture supernatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0311-BV

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant Bovine BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

Synonyms

Bone morphogenetic protein 2 ; BMP2

Gene Name

BMP2

Gene ID

615037

UniProt

C8C060

Reactivity

Bovine

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q283-R396

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Cytoskeleton/ECM, Extracellular Matrix, Growth Factors, Growth Factors/Hormones, Neural Stem Cells, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, Structures, TGF Beta

Standard

10 ng/Tube

Sample Type

Bone tissue, cell culture supernatants and serum.

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 285 742 1197 306 834 1358 Standard deviation 9.12 23.17 37.12 18.97 58.38 97.78 CV (%) 3.2 3.5 3.1 6.2 7 7.2 Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 285 742 1197 306 834 1358 Standard deviation 9.12 23.17 37.12 18.97 58.38 97.78 CV (%) 3.2 3.5 3.1 6.2 7 7.2 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 285 742 1197 306 834 1358 Mean (pg/ml) 285 742 1197 306 834 1358 Standard deviation 9.12 23.17 37.12 18.97 58.38 97.78 Standard deviation 9.12 23.17 37.12 18.97 58.38 97.78 CV (%) 3.2 3.5 3.1 6.2 7 7.2 CV (%) 3.2 3.5 3.1 6.2 7 7.2

Sensitivity

<2 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

44702 MW

Gene Name Synonym

Bone morphogenetic protein 2

Protein Name

Bone morphogenetic protein 2

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.062 0.149 0.209 0.327 0.5 0.8 1.382 2.302 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.062 0.149 0.209 0.327 0.5 0.8 1.382 2.302 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.062 0.149 0.209 0.327 0.5 0.8 1.382 2.302 O.D. 0.062 0.149 0.209 0.327 0.5 0.8 1.382 2.302

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.