- Catalog number
- EK0981-BV
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Transforming growth factor-beta 2 (TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma (POAG) . Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured Bovine anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.
- Synonyms
- Transforming growth factor beta-2; TGF-beta-2; BSC-1 cell growth inhibitor; Cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; Polyergin; Latency-associated peptide; LAP; TGFB2
- Gene Name
- TGFB2
- Gene ID
- 534069
- UniProt
- P21214
- Reactivity
- Bovine
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A303-S414
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Developmental Biology, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Metabolism, Metabolism Processes, Organogenesis, Pathways and Processes, Response To Hypoxia, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, TGF Beta
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 221 564 1145 236 576 1095 Standard deviation 13.7 31.6 46.9 15.8 33.41 70.1 CV (%) 6.2 5.6 4.1 6.7 5.8 6.4 Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 221 564 1145 236 576 1095 Standard deviation 13.7 31.6 46.9 15.8 33.41 70.1 CV (%) 6.2 5.6 4.1 6.7 5.8 6.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 221 564 1145 236 576 1095 Mean (pg/ml) 221 564 1145 236 576 1095 Standard deviation 13.7 31.6 46.9 15.8 33.41 70.1 Standard deviation 13.7 31.6 46.9 15.8 33.41 70.1 CV (%) 6.2 5.6 4.1 6.7 5.8 6.4 CV (%) 6.2 5.6 4.1 6.7 5.8 6.4
- Sensitivity
- <10 pg/mL
- Function
- TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 47748 MW
- Anticoagulant
- Heparin, EDTA or citrate
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the TGF-beta family.
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.16 0.258 0.403 0.681 1.114 1.777 3.01 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.16 0.258 0.403 0.681 1.114 1.777 3.01 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.16 0.258 0.403 0.681 1.114 1.777 3.01 O.D. 0.03 0.16 0.258 0.403 0.681 1.114 1.777 3.01
- Activating Reagent
- Solution A: 2Solution B: 22
- Sample Activation Procedure
- Activate the sample Cell culture supernatants: add activating reagent pro rata, i.e. add 20μl of Solution A into 100μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. Serum, plasma (heparin, EDTA or citrate) : add activating reagent pro rata, i.e. add 20μl of Solution A into 40??l of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. It is unnecessary to activate the recombinant TGF-beta 2. Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration. Activate the sample Cell culture supernatants: add activating reagent pro rata, i.e. add 20μl of Solution A into 100μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. Serum, plasma (heparin, EDTA or citrate) : add activating reagent pro rata, i.e. add 20μl of Solution A into 40??l of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. It is unnecessary to activate the recombinant TGF-beta 2. Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
