- Catalog number
- EK1394-PO
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.
- Synonyms
- Proepiregulin; Epiregulin; EPR; EREG
- Gene Name
- EREG
- Gene ID
- 100620969
- UniProt
- O14944
- Reactivity
- Pig
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: V60-L108
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- In normal adults, expressed predominantly in the placenta and peripheral blood leukocytes. High levels were detected in carcinomas of the bladder, lung, kidney and colon. .
- Applications
- ELISA
- Field of Research
- Cancer, Growth Factors/Hormones, Signal Transduction, Tumor Biomarkers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 3.9 pg/mL - 250 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 26.2 88.7 144 25.4 91.5 151 Standard deviation 1.18 7.94 6.81 1.73 7.05 11.93 CV (%) 4.5 5.8 5.7 6.8 7.7 7.9 Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 26.2 88.7 144 25.4 91.5 151 Standard deviation 1.18 7.94 6.81 1.73 7.05 11.93 CV (%) 4.5 5.8 5.7 6.8 7.7 7.9 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 26.2 88.7 144 25.4 91.5 151 Mean (pg/ml) 26.2 88.7 144 25.4 91.5 151 Standard deviation 1.18 7.94 6.81 1.73 7.05 11.93 Standard deviation 1.18 7.94 6.81 1.73 7.05 11.93 CV (%) 4.5 5.8 5.7 6.8 7.7 7.9 CV (%) 4.5 5.8 5.7 6.8 7.7 7.9
- Sensitivity
- <10 pg/mL
- Function
- Ligand of the EGF receptor/EGFR and ERBB4. Stimulates EGFR and ERBB4 tyrosine phosphorylation (PubMed:9419975) . Contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis and vascular remodeling and by stimulating cell proliferation (PubMed:24631357) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 19044 MW
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Proepiregulin
- Subcellular Location
- Epiregulin: Secreted, extracellular space .
- Protein Name
- Proepiregulin
- Sample Data
- Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.042 0.107 0.16 0.271 0.464 0.911 1.573 2.256 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.042 0.107 0.16 0.271 0.464 0.911 1.573 2.256 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.042 0.107 0.16 0.271 0.464 0.911 1.573 2.256 O.D. 0.042 0.107 0.16 0.271 0.464 0.911 1.573 2.256
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
