Monkey primate FGF7/KGF ELISA Kit PicoKine®

About this product

Monkey primate FGF7/KGF ELISA Kit PicoKine® (96 Tests) . Quantitate Monkey FGF7 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0820-PR

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of monkey primate and monkey primate homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.

Synonyms

Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; FGF7; KGF

Gene Name

FGF7

Gene ID

574345

UniProt

P21781

Reactivity

Monkey

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: C32-T194

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Epithelial cell.

Applications

ELISA

Field of Research

Growth Factors, Growth Factors/Hormones, Organogenesis, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 156 645 1278 147 649 1226 Standard deviation 6.40 27.09 70.29 12.35 39.59 78.46 CV (%) 4.1 4.2 5.6 8.4 6.1 6.4 Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 156 645 1278 147 649 1226 Standard deviation 6.40 27.09 70.29 12.35 39.59 78.46 CV (%) 4.1 4.2 5.6 8.4 6.1 6.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 156 645 1278 147 649 1226 Mean (pg/ml) 156 645 1278 147 649 1226 Standard deviation 6.40 27.09 70.29 12.35 39.59 78.46 Standard deviation 6.40 27.09 70.29 12.35 39.59 78.46 CV (%) 4.1 4.2 5.6 8.4 6.1 6.4 CV (%) 4.1 4.2 5.6 8.4 6.1 6.4

Sensitivity

<4 pg/mL

Function

Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

22509 MW

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Fibroblast growth factor 7

Subcellular Location

Secreted.

Protein Name

Fibroblast growth factor 7

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.013 0.093 0.18 0.335 0.581 0.995 1.51 2.005 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.013 0.093 0.18 0.335 0.581 0.995 1.51 2.005 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.013 0.093 0.18 0.335 0.581 0.995 1.51 2.005 O.D. 0.013 0.093 0.18 0.335 0.581 0.995 1.51 2.005

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.