Monkey primate Epiregulin ELISA Kit PicoKine®

About this product

Monkey primate Epiregulin ELISA Kit PicoKine® (96 Tests) . Quantitate Monkey EREG in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 0.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1394-PR

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.

Synonyms

Proepiregulin; Epiregulin; EPR; EREG

Gene Name

EREG

Gene ID

702502

UniProt

O14944

Reactivity

Monkey

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V60-L108

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

In normal adults, expressed predominantly in the placenta and peripheral blood leukocytes. High levels were detected in carcinomas of the bladder, lung, kidney and colon. .

Applications

ELISA

Field of Research

Cancer, Growth Factors/Hormones, Signal Transduction, Tumor Biomarkers

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

3.9 pg/mL - 250 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 26.2 88.7 144 25.4 91.5 151 Standard deviation 1.18 7.94 6.81 1.73 7.05 11.93 CV (%) 4.5 5.8 5.7 6.8 7.7 7.9 Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 26.2 88.7 144 25.4 91.5 151 Standard deviation 1.18 7.94 6.81 1.73 7.05 11.93 CV (%) 4.5 5.8 5.7 6.8 7.7 7.9 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 26.2 88.7 144 25.4 91.5 151 Mean (pg/ml) 26.2 88.7 144 25.4 91.5 151 Standard deviation 1.18 7.94 6.81 1.73 7.05 11.93 Standard deviation 1.18 7.94 6.81 1.73 7.05 11.93 CV (%) 4.5 5.8 5.7 6.8 7.7 7.9 CV (%) 4.5 5.8 5.7 6.8 7.7 7.9

Sensitivity

<10 pg/mL

Function

Ligand of the EGF receptor/EGFR and ERBB4. Stimulates EGFR and ERBB4 tyrosine phosphorylation (PubMed:9419975) . Contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis and vascular remodeling and by stimulating cell proliferation (PubMed:24631357) . .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

19044 MW

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Proepiregulin

Subcellular Location

Epiregulin: Secreted, extracellular space .

Protein Name

Proepiregulin

Sample Data

Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.028 0.095 0.134 0.21 0.412 0.743 1.365 2.188 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.028 0.095 0.134 0.21 0.412 0.743 1.365 2.188 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.028 0.095 0.134 0.21 0.412 0.743 1.365 2.188 O.D. 0.028 0.095 0.134 0.21 0.412 0.743 1.365 2.188

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.