- Catalog number
- EK1528
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Peroxiredoxin-2 is a protein that in humans is encoded by the PRDX2 gene. This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein plays an antioxidant protective role in cells, and it may contribute to the antiviral activity of CD8 (+) T-cells. The crystal structure of this protein has been resolved to 2.7 angstroms. This protein prevents hemolytic anemia from oxidative stress by stabilizing hemoglobin, thus making this gene a therapeutic target for patients with hemolytic anemia. It may have a proliferative effect and play a role in cancer development or progression. Related pseudogenes have been identified on chromosomes 5, 6, 10 and 13.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 405 416 419 462 425 21.7 5.1% Sample 2 3122 3014 3140 2995 3067 63.92 2% Sample 3 9534 8957 10276 9586 9588 467.55 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 405 416 419 462 425 21.7 5.1% Sample 1 405 416 419 462 425 21.7 5.1% Sample 2 3122 3014 3140 2995 3067 63.92 2% Sample 2 3122 3014 3140 2995 3067 63.92 2% Sample 3 9534 8957 10276 9586 9588 467.55 4.8% Sample 3 9534 8957 10276 9586 9588 467.55 4.8%
- Synonyms
- Peroxiredoxin-2;1.11.1.15; Natural killer cell-enhancing factor B; NKEF-B; PRP; Thiol-specific antioxidant protein; TSA; Thioredoxin peroxidase 1; Thioredoxin-dependent peroxide reductase 1; PRDX2; NKEFB, TDPX1
- Gene Name
- PRDX2
- Gene ID
- 7001
- UniProt
- P32119
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A2-N198
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Alzheimer's Disease, Cancer, Cancer Metabolism, Cell Biology, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Oxidative Stress, Pathways and Processes, Redox Metabolism
- Standard
- 20 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 312 pg/mL - 20,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 405 3122 44534 445 3416 9285 Standard deviation 22.27 140.49 638.77 27.14 198.12 724.23 CV (%) 5.5% 4.5% 6.7% 6.1% 5.8% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 405 3122 44534 445 3416 9285 Standard deviation 22.27 140.49 638.77 27.14 198.12 724.23 CV (%) 5.5% 4.5% 6.7% 6.1% 5.8% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 405 3122 44534 445 3416 9285 Mean (pg/ml) 405 3122 44534 445 3416 9285 Standard deviation 22.27 140.49 638.77 27.14 198.12 724.23 Standard deviation 22.27 140.49 638.77 27.14 198.12 724.23 CV (%) 5.5% 4.5% 6.7% 6.1% 5.8% 7.8% CV (%) 5.5% 4.5% 6.7% 6.1% 5.8% 7.8%
- Sensitivity
- <10 pg/mL
- Function
- Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H (2) O (2) .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 21892 MW
- Specificity
- Natural and recombinant human Peroxiredoxin 2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Peroxiredoxin-2
- Subcellular Location
- Cytoplasm.
- Protein Name
- Peroxiredoxin-2
- Sample Data
- Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.028 0.08 0.141 0.247 0.456 0.872 1.641 2.585 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.028 0.08 0.141 0.247 0.456 0.872 1.641 2.585 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.028 0.08 0.141 0.247 0.456 0.872 1.641 2.585 O.D. 0.028 0.08 0.141 0.247 0.456 0.872 1.641 2.585
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
