- Catalog number
- EK1487
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Afamin is a protein that in humans is encoded by the AFM gene. This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 221 236 257 237 237 12.79 5.3% Sample 2 658 655 603 647 640 22.16 3.4% Sample 3 3779 3418 3836 3710 3685 160.89 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 221 236 257 237 237 12.79 5.3% Sample 1 221 236 257 237 237 12.79 5.3% Sample 2 658 655 603 647 640 22.16 3.4% Sample 2 658 655 603 647 640 22.16 3.4% Sample 3 3779 3418 3836 3710 3685 160.89 4.3% Sample 3 3779 3418 3836 3710 3685 160.89 4.3%
- Synonyms
- Afamin; Alpha-albumin; Alpha-Alb; AFM; ALB2, ALBA
- Gene Name
- AFM
- Gene ID
- 173
- UniProt
- P43652
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L22-N599
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- High level detected in plasma but also in extravascular fluids such as follicular and cerebrospinal fluids (at protein level) . .
- Applications
- ELISA
- Field of Research
- Cardiovascular, Cofactors, Vitamins/Minerals, Metabolism, Pathways and Processes, Serum Proteins, Signal Transduction, Vitamins/Minerals
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and urine.
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 221 658 3779 249 672 4128 Standard deviation 10.83 33.56 219.18 17.68 51.07 321.98 CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 221 658 3779 249 672 4128 Standard deviation 10.83 33.56 219.18 17.68 51.07 321.98 CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 221 658 3779 249 672 4128 Mean (pg/ml) 221 658 3779 249 672 4128 Standard deviation 10.83 33.56 219.18 17.68 51.07 321.98 Standard deviation 10.83 33.56 219.18 17.68 51.07 321.98 CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8% CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8%
- Sensitivity
- <10 pg/mL
- Function
- Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the regulation and transport of vitamin E at the blood-brain barrier. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 69069 MW
- Specificity
- Natural and recombinant human Afamin
- Gene Name Synonym
- Afamin
- Subcellular Location
- Secreted.
- Protein Name
- Afamin
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
