- Catalog number
- EK1581
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- SCFR (Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 236 256 258 231 245 11.9 4.8% Sample 2 1951 1726 1646 1670 1748 120.6 6.8% Sample 3 5607 5964 6206 6301 6019 267.98 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 236 256 258 231 245 11.9 4.8% Sample 1 236 256 258 231 245 11.9 4.8% Sample 2 1951 1726 1646 1670 1748 120.6 6.8% Sample 2 1951 1726 1646 1670 1748 120.6 6.8% Sample 3 5607 5964 6206 6301 6019 267.98 4.4% Sample 3 5607 5964 6206 6301 6019 267.98 4.4%
- Synonyms
- Mast/stem cell growth factor receptor Kit; SCFR;2.7.10.1; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; CD117; Kit; Sl
- Gene Name
- Kit
- Gene ID
- 16590
- UniProt
- P05532
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S25-P527
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Isoform 1 and isoform 2 are detected in bone marrow cells, spermatogonia and spermatocytes, but not in round spermatids, elongating spermatids and spermatozoa. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa, but not in spermatogonia and spermatocytes (at protein level) . Isoform 1 is widely expressed and detected in fetal liver and bone marrow. Isoform 3 is detected in bone marrow cells enriched in hematopoietic stem cells. .
- Applications
- ELISA
- Field of Research
- Cancer, Cell Type Markers, Cytokines, Developmental Biology, Germ Cell Markers, Germline Stem Cells, Hematopoietic Progenitors, Immunology, Myeloid, Neuroscience, Neurotransmission, Oncoproteins, Oncoproteins/Suppressors, Organogenesis, Protein Phosphorylation, Receptor Tyrosine Kinases, Receptors / Channels, Reproduction, Signal Transduction, Stem Cells, Surface Molecules, Tags & Cell Markers, Tumor Associated, Tyrosine Kinases
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 236 12561 5607 256 1869 5510 Standard deviation 15.81 85.84 409.31 20.48 89.71 497.3% CV (%) 6.7% 4.4% 7.3% 8% 4.8% 9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 236 12561 5607 256 1869 5510 Standard deviation 15.81 85.84 409.31 20.48 89.71 497.3% CV (%) 6.7% 4.4% 7.3% 8% 4.8% 9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 236 12561 5607 256 1869 5510 Mean (pg/ml) 236 12561 5607 256 1869 5510 Standard deviation 15.81 85.84 409.31 20.48 89.71 497.3% Standard deviation 15.81 85.84 409.31 20.48 89.71 497.3% CV (%) 6.7% 4.4% 7.3% 8% 4.8% 9% CV (%) 6.7% 4.4% 7.3% 8% 4.8% 9%
- Sensitivity
- <10 ng/mL
- Function
- Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 109343 MW
- Specificity
- Natural and recombinant mouse CD117
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Mast/stem cell growth factor receptor Kit
- Subcellular Location
- Isoform 1: Cell membrane; Single-pass type I membrane protein.
- Protein Name
- Mast/stem cell growth factor receptor Kit
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.076 0.143 0.253 0.47 0.757 1.246 1.833 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.076 0.143 0.253 0.47 0.757 1.246 1.833 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.076 0.143 0.253 0.47 0.757 1.246 1.833 O.D. 0.011 0.076 0.143 0.253 0.47 0.757 1.246 1.833
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
