- Catalog number
- EK1586
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 150 154 170 150 156 8.24 5.2% Sample 2 602 649 645 637 633 18.55 2.9% Sample 3 1476 1672 1722 1744 1653 105.74 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 150 154 170 150 156 8.24 5.2% Sample 1 150 154 170 150 156 8.24 5.2% Sample 2 602 649 645 637 633 18.55 2.9% Sample 2 602 649 645 637 633 18.55 2.9% Sample 3 1476 1672 1722 1744 1653 105.74 6.3% Sample 3 1476 1672 1722 1744 1653 105.74 6.3%
- Synonyms
- Kallikrein-1;3.4.21.35; Glandular kallikrein K1; KAL-B; Renal kallikrein; Tissue kallikrein-6; mGK-6; Klk1; Klk-6, Klk6
- Gene Name
- Klk1
- Gene ID
- 16612
- UniProt
- P15947
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: I25-D261
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Serum Proteins, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 150 602 1476 154 580 1608 Standard deviation 9.15 43.94 116.6 11.08 51.62 136.68 CV (%) 6.1% 7.3% 7.9% 7.2% 8.9% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 150 602 1476 154 580 1608 Standard deviation 9.15 43.94 116.6 11.08 51.62 136.68 CV (%) 6.1% 7.3% 7.9% 7.2% 8.9% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 150 602 1476 154 580 1608 Mean (pg/ml) 150 602 1476 154 580 1608 Standard deviation 9.15 43.94 116.6 11.08 51.62 136.68 Standard deviation 9.15 43.94 116.6 11.08 51.62 136.68 CV (%) 6.1% 7.3% 7.9% 7.2% 8.9% 8.5% CV (%) 6.1% 7.3% 7.9% 7.2% 8.9% 8.5%
- Sensitivity
- <12 pg/mL
- Function
- Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 28775 MW
- Specificity
- Natural and recombinant mouse KLK1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Kallikrein-1
- Protein Name
- Kallikrein-1
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.122 0.184 0.309 0.529 0.881 1.455 2.069 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.122 0.184 0.309 0.529 0.881 1.455 2.069 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.122 0.184 0.309 0.529 0.881 1.455 2.069 O.D. 0.007 0.122 0.184 0.309 0.529 0.881 1.455 2.069
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
