- Catalog number
- EK0968
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Sclerostin is a protein that in humans is encoded by the SOST gene. It is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Additionally, Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 89 84 85 97 88 5.11 5.8% Sample 2 215 183 209 209 204 12.36 6% Sample 3 883 875 1058 953 942 73.39 7.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 89 84 85 97 88 5.11 5.8% Sample 1 89 84 85 97 88 5.11 5.8% Sample 2 215 183 209 209 204 12.36 6% Sample 2 215 183 209 209 204 12.36 6% Sample 3 883 875 1058 953 942 73.39 7.7% Sample 3 883 875 1058 953 942 73.39 7.7%
- Synonyms
- Sclerostin; SOST; UNQ2976/PRO7455/PRO7476
- Gene Name
- SOST
- Gene ID
- 50964
- UniProt
- Q9BQB4
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q24-Y213
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days. Detected in the subendothelial layer of the aortic intima (at protein level) . .
- Applications
- ELISA
- Field of Research
- Cytoskeleton/ECM, Developmental Biology, Extracellular Matrix, Growth Factors/Hormones, Metabolism, Organogenesis, Secreted, Signal Transduction, Signaling Pathways, Skeletal Development, Stem Cells, Structures
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 215 883 90 209 912 Standard deviation 6.75 8.6 59.16 6.74 10.65 70.22 CV (%) 6.7% 4% 6.7% 6.6% 7.7% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 215 883 90 209 912 Standard deviation 6.75 8.6 59.16 6.74 10.65 70.22 CV (%) 6.7% 4% 6.7% 6.6% 7.7% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 89 215 883 90 209 912 Mean (pg/ml) 89 215 883 90 209 912 Standard deviation 6.75 8.6 59.16 6.74 10.65 70.22 Standard deviation 6.75 8.6 59.16 6.74 10.65 70.22 CV (%) 6.7% 4% 6.7% 6.6% 7.7% 7.7% CV (%) 6.7% 4% 6.7% 6.6% 7.7% 7.7%
- Sensitivity
- <10 pg/mL
- Function
- Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 24031 MW
- Specificity
- Natural and recombinant human Sclerostin
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Sclerostin
- Subcellular Location
- Secreted, extracellular space, extracellular matrix .
- Protein Name
- Sclerostin
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.077 0.126 0.209 0.34 0.571 0.993 1.835 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.077 0.126 0.209 0.34 0.571 0.993 1.835 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.077 0.126 0.209 0.34 0.571 0.993 1.835 O.D. 0.017 0.077 0.126 0.209 0.34 0.571 0.993 1.835
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
