Human BCHE/Butyrylcholinesterase ELISA Kit PicoKine®

About this product

Human BCHE/Butyrylcholinesterase ELISA Kit PicoKine® (96 Tests) . Quantitate Human BCHE in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1507

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Butyrylcholinesterase is a nonspecific cholinesterase enzyme that in humans is encoded by the BCHE gene. This gene is mapped to 3q26. It is a member of the type-B carboxylesterase/lipase family of proteins. The encoded enzyme exhibits broad substrate specificity and is involved in the detoxification of poisons including organophosphate nerve agents and pesticides, and the metabolism of drugs including cocaine, heroin and aspirin. Humans homozygous for certain mutations in this gene exhibit prolonged apnea after administration of the muscle relaxant succinylcholine.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1205 1122 1225 1228 1195 43.06 3.6% Sample 2 4078 4285 4341 4276 4245 99.58 2.3% Sample 3 24826 24993 26084 24591 25123 572.63 2.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1205 1122 1225 1228 1195 43.06 3.6% Sample 1 1205 1122 1225 1228 1195 43.06 3.6% Sample 2 4078 4285 4341 4276 4245 99.58 2.3% Sample 2 4078 4285 4341 4276 4245 99.58 2.3% Sample 3 24826 24993 26084 24591 25123 572.63 2.2% Sample 3 24826 24993 26084 24591 25123 572.63 2.2%

Synonyms

Cholinesterase;3.1.1.8; Acylcholine acylhydrolase; Butyrylcholine esterase; Choline esterase II; Pseudocholinesterase; BCHE; CHE1

Gene Name

BCHE

Gene ID

590

UniProt

P06276

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E29-L602

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Detected in blood plasma (at protein level) . Present in most cells except erythrocytes. .

Applications

ELISA

Field of Research

Alzheimer's Disease, Drug Metabolism, Metabolic Signaling Pathways, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Neurotransmitter, Pathways and Processes, Signal Transduction

Standard

40 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.

Detection Range

625 pg/mL - 40,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1205 4078 24826 1297 4376 24447 Standard deviation 66.27 322.16 1613.69 80.41 380.71 1662.39 CV (%) 5.5% 7.9% 6.5% 6.2% 8.7% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1205 4078 24826 1297 4376 24447 Standard deviation 66.27 322.16 1613.69 80.41 380.71 1662.39 CV (%) 5.5% 7.9% 6.5% 6.2% 8.7% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1205 4078 24826 1297 4376 24447 Mean (pg/ml) 1205 4078 24826 1297 4376 24447 Standard deviation 66.27 322.16 1613.69 80.41 380.71 1662.39 Standard deviation 66.27 322.16 1613.69 80.41 380.71 1662.39 CV (%) 5.5% 7.9% 6.5% 6.2% 8.7% 6.8% CV (%) 5.5% 7.9% 6.5% 6.2% 8.7% 6.8%

Sensitivity

<10 pg/mL

Function

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

68418 MW

Specificity

Natural and recombinant human BCHE

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cholinesterase

Subcellular Location

Secreted .

Protein Name

Cholinesterase

Sample Data

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.046 0.111 0.215 0.357 0.6255 1.063 1.582 1.999 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.046 0.111 0.215 0.357 0.6255 1.063 1.582 1.999 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.046 0.111 0.215 0.357 0.6255 1.063 1.582 1.999 O.D. 0.046 0.111 0.215 0.357 0.6255 1.063 1.582 1.999

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.