- Catalog number
- EK1610
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP) -9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 39 39 35 34 36 2.27 6.3% Sample 2 220 263 251 256 247 16.43 6.6% Sample 3 419 420 444 379 415 23.32 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 39 39 35 34 36 2.27 6.3% Sample 1 39 39 35 34 36 2.27 6.3% Sample 2 220 263 251 256 247 16.43 6.6% Sample 2 220 263 251 256 247 16.43 6.6% Sample 3 419 420 444 379 415 23.32 5.6% Sample 3 419 420 444 379 415 23.32 5.6%
- Synonyms
- Growth/differentiation factor 2; GDF-2; Bone morphogenetic protein 9; BMP-9; Gdf2; Bmp9
- Gene Name
- Gdf2
- Gene ID
- 12165
- UniProt
- Q9WV56
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: S319-R428
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cell Biology, Cell Cycle, Cell Differentiation, Cytoskeleton/ECM, Extracellular Matrix, Growth Factors/Hormones, Signal Transduction, Structures
- Standard
- 10 ng/Tube
- Sample Type
- Bone tissue, cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 39 220 419 41 226 402 Standard deviation 1.71 11.22 31.42 2.05 14.46 30.95 CV (%) 4.4% 7.7%% 7.5% 5% 6.4% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 39 220 419 41 226 402 Standard deviation 1.71 11.22 31.42 2.05 14.46 30.95 CV (%) 4.4% 7.7%% 7.5% 5% 6.4% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 39 220 419 41 226 402 Mean (pg/ml) 39 220 419 41 226 402 Standard deviation 1.71 11.22 31.42 2.05 14.46 30.95 Standard deviation 1.71 11.22 31.42 2.05 14.46 30.95 CV (%) 4.4% 7.7%% 7.5% 5% 6.4% 7.7% CV (%) 4.4% 7.7%% 7.5% 5% 6.4% 7.7%
- Sensitivity
- <10 pg/mL
- Function
- Potent circulating inhibitor of angiogenesis. Could be involved in bone formation. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse BMP-9
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Growth differentiation factor 2
- Subcellular Location
- Secreted.
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.046 0.133 0.215 0.367 0.657 1.006 1.669 2.392 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.046 0.133 0.215 0.367 0.657 1.006 1.669 2.392 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.046 0.133 0.215 0.367 0.657 1.006 1.669 2.392 O.D. 0.046 0.133 0.215 0.367 0.657 1.006 1.669 2.392
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
