Human IL-23R/Interleukin-23 receptor ELISA Kit PicoKine®

About this product

Human IL-23R ELISA Kit PicoKine® (96 Tests) . Quantitate Human IL23R in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1612

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-23 subunit alpha is a protein that in humans is encoded by the IL23A gene. The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 355 334 354 307 337 19.5 5.7% Sample 2 1260 1269 1408 1249 1296 64.76 4.9% Sample 3 6219 5300 5785 5269 5643 390.34 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 355 334 354 307 337 19.5 5.7% Sample 1 355 334 354 307 337 19.5 5.7% Sample 2 1260 1269 1408 1249 1296 64.76 4.9% Sample 2 1260 1269 1408 1249 1296 64.76 4.9% Sample 3 6219 5300 5785 5269 5643 390.34 6.9% Sample 3 6219 5300 5785 5269 5643 390.34 6.9%

Synonyms

Interleukin-23 receptor; IL-23 receptor; IL-23R; IL23R

Gene Name

IL23R

Gene ID

149233

UniProt

Q5VWK5

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I25-I354

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed by monocytes, Th1, Th0, NK and dendritic cells. Isoform 1 is specifically expressed in NK cells.

Applications

ELISA

Field of Research

Cell Type Markers, Cytokines, Epigenetics and Nuclear Signaling, Immunoglobulins, Immunology, Innate Immunity, Interleukins, Nuclear Signaling, Nuclear Signaling Pathways, Receptors, Signal Transduction, Signaling Pathway

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 355 1260 6219 384 1339 6060 Standard deviation 19.52 79.38 478.86 28.03 99.08 545.40 CV (%) 5.5% 7.4% 7.7% 7.3% 7.4% 9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 355 1260 6219 384 1339 6060 Standard deviation 19.52 79.38 478.86 28.03 99.08 545.40 CV (%) 5.5% 7.4% 7.7% 7.3% 7.4% 9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 355 1260 6219 384 1339 6060 Mean (pg/ml) 355 1260 6219 384 1339 6060 Standard deviation 19.52 79.38 478.86 28.03 99.08 545.40 Standard deviation 19.52 79.38 478.86 28.03 99.08 545.40 CV (%) 5.5% 7.4% 7.7% 7.3% 7.4% 9% CV (%) 5.5% 7.4% 7.7% 7.3% 7.4% 9%

Sensitivity

<10 pg/mL

Function

Associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human IL-23R

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin 23 receptor

Subcellular Location

Cell membrane

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.064 0.187 0.275 0.416 0.6356 1.057 1.591 2.085 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.064 0.187 0.275 0.416 0.6356 1.057 1.591 2.085 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.064 0.187 0.275 0.416 0.6356 1.057 1.591 2.085 O.D. 0.064 0.187 0.275 0.416 0.6356 1.057 1.591 2.085

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.