Mouse Cardiotrophin-1 / CT-1 ELISA Kit PicoKine®

About this product

Mouse Cardiotrophin-1 / CT-1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Ctf1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0564

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cardiotrophin-1 (CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), interleukin-6 (IL6), and interleukin-11 (IL11) . And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80% amino acid identity with the 203-amino acid mouse CT-1 sequence; however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidney.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 270 247 263 274 263 10.3 3.9% Sample 2 1852 1876 1650 1677 1763 101.06 5.7% Sample 3 6181 6219 5425 5308 5783 419.01 7.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 270 247 263 274 263 10.3 3.9% Sample 1 270 247 263 274 263 10.3 3.9% Sample 2 1852 1876 1650 1677 1763 101.06 5.7% Sample 2 1852 1876 1650 1677 1763 101.06 5.7% Sample 3 6181 6219 5425 5308 5783 419.01 7.2% Sample 3 6181 6219 5425 5308 5783 419.01 7.2%

Synonyms

Cardiotrophin-1; CT-1; Ctf1; CT1; CT 1

Gene Name

Ctf1

Gene ID

13019

UniProt

Q60753

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S2-A203

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels in testis and brain. No expression in spleen.

Applications

ELISA

Field of Research

Atherosclerosis, Cardiovascular, Cytokines, Diabetes-associated, Energy Metabolism, Energy Transfer Pathways, Hypertrophy, Immunology, Innate Immunity, Lipid Metabolism, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 270 1852 6181 243 1959 6792 Standard deviation 17.55 79.63 438.85 16.03 84.23 584.11 CV (%) 6.5% 4.3% 7.1% 6.6% 4.3% 8.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 270 1852 6181 243 1959 6792 Standard deviation 17.55 79.63 438.85 16.03 84.23 584.11 CV (%) 6.5% 4.3% 7.1% 6.6% 4.3% 8.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 270 1852 6181 243 1959 6792 Mean (pg/ml) 270 1852 6181 243 1959 6792 Standard deviation 17.55 79.63 438.85 16.03 84.23 584.11 Standard deviation 17.55 79.63 438.85 16.03 84.23 584.11 CV (%) 6.5% 4.3% 7.1% 6.6% 4.3% 8.6% CV (%) 6.5% 4.3% 7.1% 6.6% 4.3% 8.6%

Sensitivity

<10 pg/mL

Function

Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse CT-1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cardiotrophin 1

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.072 0.116 0.197 0.334 0.578 1.037 1.932 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.072 0.116 0.197 0.334 0.578 1.037 1.932 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.072 0.116 0.197 0.334 0.578 1.037 1.932 O.D. 0.026 0.072 0.116 0.197 0.334 0.578 1.037 1.932

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.