- Catalog number
- EK1615
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. In humans, clusterin is encoded by the CLU gene.This protein has several synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI) . Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 428 422 418 423 422 3.56 0.8% Sample 2 2367 2282 2285 2383 2329 46.11 1.9% Sample 3 10471 10267 10049 10442 10307 168.28 1.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 428 422 418 423 422 3.56 0.8% Sample 1 428 422 418 423 422 3.56 0.8% Sample 2 2367 2282 2285 2383 2329 46.11 1.9% Sample 2 2367 2282 2285 2383 2329 46.11 1.9% Sample 3 10471 10267 10049 10442 10307 168.28 1.6% Sample 3 10471 10267 10049 10442 10307 168.28 1.6%
- Synonyms
- Clusterin; Dimeric acid glycoprotein; DAG; Sulfated glycoprotein 2; SGP-2; Testosterone repressed prostate message 2; TRPM-2; Clusterin beta chain; Clusterin alpha chain; Clu
- Gene Name
- Clu
- Gene ID
- 24854
- UniProt
- P05371
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: D22-R226 (β) & S227-E448 (α)
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Detected in Sertoli cells (at protein level) . Detected in cultured Sertoli cells, testis, epididymis, liver and brain.
- Applications
- ELISA
- Field of Research
- Cancer, Cardiovascular, Chaperones, Complement, Immunology, Innate Immunity, Lipid Metabolism, Lipids/Lipoproteins, Metabolism, Protein Trafficking, Signal Transduction, Tumor Biomarkers
- Standard
- 20 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 312 pg/mL - 20,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 428 2367 10471 423 2436 10928 Standard deviation 19.26 120.71 827.2 21.57 160.77 1060.01 CV (%) 4.5% 9.7% 7.9% 9.7% 6.6% 9.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 428 2367 10471 423 2436 10928 Standard deviation 19.26 120.71 827.2 21.57 160.77 1060.01 CV (%) 4.5% 9.7% 7.9% 9.7% 6.6% 9.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 428 2367 10471 423 2436 10928 Mean (pg/ml) 428 2367 10471 423 2436 10928 Standard deviation 19.26 120.71 827.2 21.57 160.77 1060.01 Standard deviation 19.26 120.71 827.2 21.57 160.77 1060.01 CV (%) 4.5% 9.7% 7.9% 9.7% 6.6% 9.7% CV (%) 4.5% 9.7% 7.9% 9.7% 6.6% 9.7%
- Sensitivity
- <15 pg/mL
- Function
- Functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro) . Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Promotes apoptosis when in the nucleus. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant rat Clusterin
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Clusterin
- Subcellular Location
- Secreted
- Sample Data
- Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.042 0.142 0.218 0.363 0.65 1.061 1.613 2.046 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.042 0.142 0.218 0.363 0.65 1.061 1.613 2.046 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.042 0.142 0.218 0.363 0.65 1.061 1.613 2.046 O.D. 0.042 0.142 0.218 0.363 0.65 1.061 1.613 2.046
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
