Mouse PON1/Paraoxonase 1 ELISA Kit PicoKine®

About this product

Mouse PON1/Paraoxonase 1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Pon1 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1621

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serum paraoxonase/arylesterase 1 (PON1) also known as aromatic esterase 1 or serum aryldialkylphosphatase 1 is an enzyme that in humans is encoded by the PON1 gene. The PON gene was mapped to chromosome 7q21-q22, and the mouse Pon1 gene was mapped to the proximal end of chromosome 6. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL) .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 364 310 330 311 328 21.85 6.6% Sample 2 1139 1282 1324 1250 1248 68.58 5.4% Sample 3 5447 5579 4941 5080 5261 260.23 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 364 310 330 311 328 21.85 6.6% Sample 1 364 310 330 311 328 21.85 6.6% Sample 2 1139 1282 1324 1250 1248 68.58 5.4% Sample 2 1139 1282 1324 1250 1248 68.58 5.4% Sample 3 5447 5579 4941 5080 5261 260.23 4.9% Sample 3 5447 5579 4941 5080 5261 260.23 4.9%

Synonyms

Serum paraoxonase/arylesterase 1; PON 1; 3.1.1.2

Gene Name

Pon1

Gene ID

18979

UniProt

P52430

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A30-L355

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Plasma, liver, kidney, heart, brain, small intestine and lung. In the plasma, associated with HDL.

Applications

ELISA

Field of Research

Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Diabetes, Diabetes-associated, Drug Metabolism, Heart Disease, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Oxidative Stress, Pathways and Processes, Redox Metabolism, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 364 1139 5447 343 1198 5787 Standard deviation 21.47 54.67 403.07 25.38 79.06 468.74 CV (%) 7.4% 4.8% 7.4% 7.4% 6.6% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 364 1139 5447 343 1198 5787 Standard deviation 21.47 54.67 403.07 25.38 79.06 468.74 CV (%) 7.4% 4.8% 7.4% 7.4% 6.6% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 364 1139 5447 343 1198 5787 Mean (pg/ml) 364 1139 5447 343 1198 5787 Standard deviation 21.47 54.67 403.07 25.38 79.06 468.74 Standard deviation 21.47 54.67 403.07 25.38 79.06 468.74 CV (%) 7.4% 4.8% 7.4% 7.4% 6.6% 8.1% CV (%) 7.4% 4.8% 7.4% 7.4% 6.6% 8.1%

Sensitivity

<10 pg/mL

Function

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse PON1

Anticoagulant

Heparin

Gene Name Synonym

Paraoxonase 1

Subcellular Location

Secreted, extracellular space.

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.048 0.127 0.203 0.365 0.624 1.001 1.467 2.001 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.048 0.127 0.203 0.365 0.624 1.001 1.467 2.001 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.048 0.127 0.203 0.365 0.624 1.001 1.467 2.001 O.D. 0.048 0.127 0.203 0.365 0.624 1.001 1.467 2.001

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.