Human PD-L2/B7-DC ELISA Kit PicoKine®

About this product

Human PD-L2/B7-DC ELISA Kit PicoKine® (96 Tests) . Quantitate Human PDCD1LG2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1447

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Programmed cell death 1 ligand 2 (also known as PD-L2, B7-DC) is a protein that in humans is encoded by the PDCD1LG2 gene. It is mapped to chromosome 9p24.2. PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immuno-receptors. Northern blot analysis detect wide expression of PDL2, with highest levels in placenta, heart, pancreas, lung, and liver, and lower levels in spleen, lymph nodes, and thymus. PDL2 negatively regulates T cells and plays an essential role in immune tolerance.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 133 126 127 117 125 5.71 4.5% Sample 2 396 453 452 415 429 24.44 5.6% Sample 3 998 945 1041 1035 1004 38.22 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 133 126 127 117 125 5.71 4.5% Sample 1 133 126 127 117 125 5.71 4.5% Sample 2 396 453 452 415 429 24.44 5.6% Sample 2 396 453 452 415 429 24.44 5.6% Sample 3 998 945 1041 1035 1004 38.22 3.8% Sample 3 998 945 1041 1035 1004 38.22 3.8%

Synonyms

Programmed cell death 1 ligand 2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; Butyrophilin B7-DC; B7-DC; CD273; PDCD1LG2; B7DC, CD273, PDCD1L2, PDL2

Gene Name

PDCD1LG2

Gene ID

80380

UniProt

Q9BQ51

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-T220

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus.

Applications

ELISA

Field of Research

Immunoglobulins, Immunology, Receptors

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 133 396 998 133 415 903 Standard deviation 7.18 28.11 57.318 7.31 37.35 54.18 CV (%) 5.4% 7.1% 5.8% 5.5% 9% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 133 396 998 133 415 903 Standard deviation 7.18 28.11 57.318 7.31 37.35 54.18 CV (%) 5.4% 7.1% 5.8% 5.5% 9% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 133 396 998 133 415 903 Mean (pg/ml) 133 396 998 133 415 903 Standard deviation 7.18 28.11 57.318 7.31 37.35 54.18 Standard deviation 7.18 28.11 57.318 7.31 37.35 54.18 CV (%) 5.4% 7.1% 5.8% 5.5% 9% 6% CV (%) 5.4% 7.1% 5.8% 5.5% 9% 6%

Sensitivity

<12 pg/mL

Function

Involved in the costimulatory signal, essential for T- cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity) . .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human PD-L2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Programmed cell death 1 ligand 2

Subcellular Location

Isoform 3: Secreted

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.