- Catalog number
- EK1619
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Notch proteins are single-pass transmembrane receptors that regulate cell fate decisions during development. The Notch family includes 4 receptors, NOTCH1, NOTCH2, NOTCH3, and NOTCH4, whose ligands include JAG1, JAG2, DLL1), DLL3, and DLL4. Notch homolog 1, translocation-associated (NOTCH1), is a human gene encoding a single-pass transmembrane receptor. It functions as a receptor for membrane bound ligands, and may play multiple roles during development. NOTCH1 may normally coordinates the process of somitogenesis, and the activated Notch 1 and Notch 3 promote differentiation of progenitor cells into astroglia.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 150 158 163 158 5.11 3.2% Sample 2 460 498 454 448 465 19.51 4.1% Sample 3 1757 1542 1704 1550 1638 94.17 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 150 158 163 158 5.11 3.2% Sample 1 162 150 158 163 158 5.11 3.2% Sample 2 460 498 454 448 465 19.51 4.1% Sample 2 460 498 454 448 465 19.51 4.1% Sample 3 1757 1542 1704 1550 1638 94.17 5.7% Sample 3 1757 1542 1704 1550 1638 94.17 5.7%
- Synonyms
- Neurogenic locus notch homolog protein 1; Notch 1; hN1; Translocation-associated notch protein TAN-1; Notch 1 extracellular truncation; NEXT; Notch 1 intracellular domain; NICD; NOTCH1; TAN1
- Gene Name
- NOTCH1
- Gene ID
- 4851
- UniProt
- P46531
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A19-Q526
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
- Applications
- ELISA
- Field of Research
- Cardiogenesis, Cardiovascular, Developmental Biology, Embryogenesis, Epigenetics and Nuclear Signaling, Hematopoietic Progenitors, Intracellular, Neural Stem Cells, Neurology Process, Neuroscience, Nuclear, Signaling Pathways, Stem Cells, Surface Molecules, Transcription, Transcription Factors/Regulators
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 460 1757 166 428 1780 Standard deviation 9.39 34.5 103.66 11.12 38.94 106.8 CV (%) 5.8% 7.5% 5.9% 6.7% 9.1% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 460 1757 166 428 1780 Standard deviation 9.39 34.5 103.66 11.12 38.94 106.8 CV (%) 5.8% 7.5% 5.9% 6.7% 9.1% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 162 460 1757 166 428 1780 Mean (pg/ml) 162 460 1757 166 428 1780 Standard deviation 9.39 34.5 103.66 11.12 38.94 106.8 Standard deviation 9.39 34.5 103.66 11.12 38.94 106.8 CV (%) 5.8% 7.5% 5.9% 6.7% 9.1% 6% CV (%) 5.8% 7.5% 5.9% 6.7% 9.1% 6%
- Sensitivity
- <12 pg/mL
- Function
- Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO) .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human NOTCH1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Notch 1
- Subcellular Location
- Cell membrane ; Single-pass type I membrane protein .
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.045 0.083 0.121 0.208 0.391 0.744 1.37 1.998 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.045 0.083 0.121 0.208 0.391 0.744 1.37 1.998 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.045 0.083 0.121 0.208 0.391 0.744 1.37 1.998 O.D. 0.045 0.083 0.121 0.208 0.391 0.744 1.37 1.998
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
