- Catalog number
- EK0731
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chemokine (C-X-C motif) ligand 7 (CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine (C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein (PBP) and connective tissue-activating peptide III (CTAP3) . It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 37 36 36 35 1.5 4.2% Sample 2 190 203 205 198 199 5.78 2.9% Sample 3 491 492 496 465 486 12.26 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 37 36 36 35 1.5 4.2% Sample 1 33 37 36 36 35 1.5 4.2% Sample 2 190 203 205 198 199 5.78 2.9% Sample 2 190 203 205 198 199 5.78 2.9% Sample 3 491 492 496 465 486 12.26 2.5% Sample 3 491 492 496 465 486 12.26 2.5%
- Synonyms
- CXC chemokine RTCK1
- Gene Name
- Ppbp
- Gene ID
- 246358
- UniProt
- Q99ME0
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: K38-Y111
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cardiovascular, Chemokines, Immunology, Innate Immunity, Platelets
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 190 491 32 177 470 Standard deviation 2.11 10.64 25.04 2.65 12.56 29.61 CV (%) 6.4% 5.6% 6.3%% 8.3% 7.1% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 190 491 32 177 470 Standard deviation 2.11 10.64 25.04 2.65 12.56 29.61 CV (%) 6.4% 5.6% 6.3%% 8.3% 7.1% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 190 491 32 177 470 Mean (pg/ml) 33 190 491 32 177 470 Standard deviation 2.11 10.64 25.04 2.65 12.56 29.61 Standard deviation 2.11 10.64 25.04 2.65 12.56 29.61 CV (%) 6.4% 5.6% 6.3%% 8.3% 7.1% 6.3% CV (%) 6.4% 5.6% 6.3%% 8.3% 7.1% 6.3%
- Sensitivity
- <2 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant rat CXCL7
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Pro-platelet basic protein
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.065 0.138 0.303 0.53 1.091 1.601 1.9 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.065 0.138 0.303 0.53 1.091 1.601 1.9 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.065 0.138 0.303 0.53 1.091 1.601 1.9 O.D. 0.001 0.065 0.138 0.303 0.53 1.091 1.601 1.9
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
