- Catalog number
- EK1111
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Inducible T-cell costimulator is an immune checkpoint protein that in humans is encoded by the ICOS gene. The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 189 189 191 192 190 1.29 0.6% Sample 2 561 545 489 526 530 26.84 5% Sample 3 1066 1100 974 1134 1068 59.62 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 189 189 191 192 190 1.29 0.6% Sample 1 189 189 191 192 190 1.29 0.6% Sample 2 561 545 489 526 530 26.84 5% Sample 2 561 545 489 526 530 26.84 5% Sample 3 1066 1100 974 1134 1068 59.62 5.5% Sample 3 1066 1100 974 1134 1068 59.62 5.5%
- Synonyms
- Inducible T-cell costimulator; Activation-inducible lymphocyte immunomediatory molecule; CD278; ICOS; AILIM
- Gene Name
- ICOS
- Gene ID
- 29851
- UniProt
- Q9Y6W8
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E21-F141
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Biotinylated polyclonal antibody from goat
- Tissue Specificity
- Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B- cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Immunology, T Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 189 561 1066 198 531 1149 Standard deviation 13.41 22.44 52.23 16.83 27.61 58.59 CV (%) 7.1% 4% 4.9% 8.5% 5.2% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 189 561 1066 198 531 1149 Standard deviation 13.41 22.44 52.23 16.83 27.61 58.59 CV (%) 7.1% 4% 4.9% 8.5% 5.2% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 189 561 1066 198 531 1149 Mean (pg/ml) 189 561 1066 198 531 1149 Standard deviation 13.41 22.44 52.23 16.83 27.61 58.59 Standard deviation 13.41 22.44 52.23 16.83 27.61 58.59 CV (%) 7.1% 4% 4.9% 8.5% 5.2% 5.1% CV (%) 7.1% 4% 4.9% 8.5% 5.2% 5.1%
- Sensitivity
- <2 pg/mL
- Function
- Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up- regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up- regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre- activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity) .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human ICOS
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Inducible T-cell costimulator
- Subcellular Location
- Isoform 1: Cell membrane ; Single-pass type I membrane protein .
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.043 0.081 0.137 0.215 0.406 0.714 1.226 1.817 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.043 0.081 0.137 0.215 0.406 0.714 1.226 1.817 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.043 0.081 0.137 0.215 0.406 0.714 1.226 1.817 O.D. 0.043 0.081 0.137 0.215 0.406 0.714 1.226 1.817
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
