- Catalog number
- EK1359
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin 24 (IL-24) is a cytokine belonging to the IL-10 family of cytokines that signals through two heterodimeric receptors: IL-20R1/IL-20R2 and IL-22R1/IL-20R2. This interleukin is also known as Melanoma differentiation-associated 7 (mda-7) due to its discovery as a tumour suppressing protein. By radiation hybrid analysis, the MDA7 gene is mapped to 1q32.2-q41. IL-24 appears to control in cell survival and proliferation by inducing rapid activation of particular transcription factors called Stat-1 and Stat-3. Mouse IL24 was expressed as an approximately 35-kD protein that was converted to a 23-kD protein by deglycosylation. The IL24 protein shares 24 to 33% homology with other members of the IL10 family of cytokines, with highest homology with IL20. This cytokine is predominantly released by activated monocytes, macrophages and T helper 2 (Th2) cells and acts on non-haematopoietic tissues such as skin, lung and reproductive tissues. IL-24 performs important roles in wound healing, psoriasis and cancer.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 142 155 146 133 144 7.9 5.4% Sample 2 555 583 584 618 585 22.32 3.8% Sample 3 1654 1616 1741 1741 1688 54.67 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 142 155 146 133 144 7.9 5.4% Sample 1 142 155 146 133 144 7.9 5.4% Sample 2 555 583 584 618 585 22.32 3.8% Sample 2 555 583 584 618 585 22.32 3.8% Sample 3 1654 1616 1741 1741 1688 54.67 3.2% Sample 3 1654 1616 1741 1741 1688 54.67 3.2%
- Synonyms
- Interleukin-24; IL-24; Melanoma differentiation-associated gene 7 protein; MDA-7; Suppression of tumorigenicity 16 protein; IL24; MDA7; ST16
- Gene Name
- Il24
- Gene ID
- 93672
- UniProt
- Q925S4
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: Q27-L181
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Biotinylated polyclonal antibody from goat
- Tissue Specificity
- Up-regulated in melanoma cells induced to terminally differentiate.
- Applications
- ELISA
- Field of Research
- Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 142 555 1654 131 526 1652 Standard deviation 8.94 25.53 77.73 5.7%%5 29.98 90.86 CV (%) 5.7%% 4.6% 4.7% 5.7%% 5.7% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 142 555 1654 131 526 1652 Standard deviation 8.94 25.53 77.73 5.7%%5 29.98 90.86 CV (%) 5.7%% 4.6% 4.7% 5.7%% 5.7% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 142 555 1654 131 526 1652 Mean (pg/ml) 142 555 1654 131 526 1652 Standard deviation 8.94 25.53 77.73 5.7%%5 29.98 90.86 Standard deviation 8.94 25.53 77.73 5.7%%5 29.98 90.86 CV (%) 5.7%% 4.6% 4.7% 5.7%% 5.7% 5.5% CV (%) 5.7%% 4.6% 4.7% 5.7%% 5.7% 5.5%
- Sensitivity
- <10 pg/mL
- Function
- Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse IL-24
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin 24
- Subcellular Location
- Secreted .
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.084 0.129 0.171 0.233 0.391 0.712 1.32 2.04 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.084 0.129 0.171 0.233 0.391 0.712 1.32 2.04 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.084 0.129 0.171 0.233 0.391 0.712 1.32 2.04 O.D. 0.084 0.129 0.171 0.233 0.391 0.712 1.32 2.04
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
